Anticorps Polyclonal de lapin anti-AKAP7

• Phare
• Validé par KD/KO

AKAP7 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 12591-1-AP

Synonymes

A kinase anchor protein 18 kDa, AKAP 18, AKAP 7 isoforms alpha and beta, AKAP18, AKAP7

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Galerie de données de validation

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  WB analysis of human brain using 12591-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12591-1-AP (AKAP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 12591-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12591-1-AP (AKAP7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12591-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12591-1-AP (AKAP7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12591-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12591-1-AP (AKAP7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12591-1-AP

  IP Result of anti-AKAP7 (IP:12591-1-AP, 4ug; Detection:12591-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of human brain using 12591-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12591-1-AP (AKAP7 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 12591-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12591-1-AP (AKAP7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 12591-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12591-1-AP (AKAP7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 12591-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12591-1-AP (AKAP7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 12591-1-AP

  Immunofluorescent analysis of HepG2 cells, using AKAP7 antibody 12591-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain,
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

12591-1-AP cible AKAP7 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AKAP7 Protéine recombinante Ag3297
• Nom complet: A kinase (PRKA) anchor protein 7
• Masse moléculaire calculée: 81 aa, 9 kDa
• Poids moléculaire observé: 11 kDa, 18 kDa, 37 kDa
• Numéro d’acquisition GenBank: BC016927
• Symbole du gène: AKAP7
• Identification du gène (NCBI): 9465
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AKAP7, also named as AKAP15 and AKAP18, localize PKA in complexes with CaV1.2 and PLN, respectively. AKAP7 targets the cAMP-dependent protein kinase (PKA) to the plasma membrane, and permits functional coupling to the L-type calcium channel. AKAP7 has short isoform AKAP7 alpha and AKAP7 beta with MW 15-18 kDa; long isoform AKAP7 gamma with MW 37-42 kDa. AKAP7alpha was highly expressed only in brain and weakly in lung lysates from WT animals. This antibody can recognize all the isoforms of AKAP7.

Publications

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; Cardiomyocytes from AKAP7 knockout mice respond normally to adrenergic stimulation.; Authors - Jones Brian W BW ; KO Validated
mouse	WB,IF	Elife; Targeted deletion of AKAP7 in dentate granule cells impairs spatial discrimination.; Authors - Brian W Jones ; KO Validated
human, mouse	WB	MBio; Murine AKAP7 Has a 2',5'-Phosphodiesterase Domain That Can Complement an Inactive Murine Coronavirus ns2 Gene.; Authors - Elona Gusho
human	WB	Clin Proteomics; iTRAQ plasma proteomics analysis for candidate biomarkers of type 2 incipient diabetic nephropathy.; Authors - Hongmei Lu

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