Anticorps Polyclonal de lapin anti-AKAP7

• Phare
• Validé par KD/KO

AKAP7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12591-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of human brain using 12591-1-AP (same clone as 12591-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12591-1-AP (AKAP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12591-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 12591-1-AP (same clone as 12591-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12591-1-AP (AKAP7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12591-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 12591-1-AP (same clone as 12591-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12591-1-AP (AKAP7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12591-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 12591-1-AP (same clone as 12591-1-PBS)

  IP result of anti-AKAP7 (IP:12591-1-AP, 4ug; Detection:12591-1-AP 1:300) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 12591-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 12591-1-AP (same clone as 12591-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12591-1-AP (AKAP7 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12591-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 12591-1-AP (same clone as 12591-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12591-1-AP (AKAP7 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12591-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 12591-1-AP (same clone as 12591-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12591-1-AP (AKAP7 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12591-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 12591-1-AP (same clone as 12591-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12591-1-AP (AKAP7 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12591-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 12591-1-AP (same clone as 12591-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using AKAP7 antibody 12591-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 12591-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12591-1-PBS cible AKAP7 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AKAP7 Protéine recombinante Ag3297
• Nom complet: A kinase (PRKA) anchor protein 7
• Masse moléculaire calculée: 81 aa, 9 kDa
• Poids moléculaire observé: 11 kDa, 18 kDa, 37 kDa
• Numéro d’acquisition GenBank: BC016927
• Symbole du gène: AKAP7
• Identification du gène (NCBI): 9465
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AKAP7, also named as AKAP15 and AKAP18, localize PKA in complexes with CaV1.2 and PLN, respectively. AKAP7 targets the cAMP-dependent protein kinase (PKA) to the plasma membrane, and permits functional coupling to the L-type calcium channel. AKAP7 has short isoform AKAP7 alpha and AKAP7 beta with MW 15-18 kDa; long isoform AKAP7 gamma with MW 37-42 kDa. AKAP7alpha was highly expressed only in brain and weakly in lung lysates from WT animals. This antibody can recognize all the isoforms of AKAP7.

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