Anticorps Polyclonal de lapin anti-AKD2

AKD2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27554-1-PBS

Synonymes

AK9, Adenylate kinase domain-containing protein 1, Adenylate kinase domain-containing protein 2, AK 9, AKD1

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Galerie de données de validation

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  WB analysis using 27554-1-AP (same clone as 27554-1-PBS)

  Various lysates lysate were subjected to SDS PAGE followed by western blot with 27554-1-AP (AKD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.

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  WB analysis of SMMC-7721 using 27554-1-AP (same clone as 27554-1-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 27554-1-AP (AKD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.

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  WB analysis of HuH-7 using 27554-1-AP (same clone as 27554-1-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 27554-1-AP (AKD2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 27554-1-AP (same clone as 27554-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27554-1-AP (AKD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 27554-1-AP (same clone as 27554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27554-1-AP (AKD2 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 27554-1-AP (same clone as 27554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27554-1-AP (AKD2 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 27554-1-AP (same clone as 27554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27554-1-AP (AKD2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 27554-1-AP (same clone as 27554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27554-1-AP (AKD2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27554-1-PBS cible AKD2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AKD2 Protéine recombinante Ag25805
• Nom complet: adenylate kinase domain containing 2
• Masse moléculaire calculée: 221 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC022031
• Symbole du gène: AKD2
• Identification du gène (NCBI): 221264
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AK9, also named as adenylate kinase 9, belongs to adenylate kinase family, which is a small, ususlly monomeric, enzyme found in every living thing due to its crucial role in energenic metabolism(PMID: 30666486). AK9 is a nucleoside mono- and diphosphate kinase due to its broad phosphatase activity, the local concentration of substrates probably influences catalytic activity(PMID: 24495878). This antibody can detect 45-50 kDa band of AK9(PMID: 23416111).

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