Anticorps Monoclonal anti-AKR1B1

• Phare
• Validé par KD/KO

AKR1B1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2F11G5

N° de cat : 67498-1-PBS

Synonymes

2F11G5, AKR1B 1, Aldo-keto reductase family 1 member B1, ALDR1, ALR2

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Galerie de données de validation

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  WB analysis of HeLa using 67498-1-Ig (same clone as 67498-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67498-1-Ig (AKR1B1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67498-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67498-1-Ig (same clone as 67498-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67498-1-Ig (AKR1B1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67498-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67498-1-Ig (same clone as 67498-1-PBS)

  WB result of AKR1B1 antibody (67498-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKR1B1 transfected HepG2 cells. This data was developed using the same antibody clone with 67498-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67498-1-Ig (same clone as 67498-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67498-1-Ig (AKR1B1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67498-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67498-1-Ig (same clone as 67498-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67498-1-Ig (AKR1B1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67498-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67498-1-Ig (same clone as 67498-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67498-1-Ig (AKR1B1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67498-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67498-1-Ig (same clone as 67498-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67498-1-Ig (AKR1B1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67498-1-PBS in a different storage buffer formulation.

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  IHC staining of human bladder using 67498-1-Ig (same clone as 67498-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 67498-1-Ig (AKR1B1 antibody) at dilution of 1:500 (under 10x lens).. This data was developed using the same antibody clone with 67498-1-PBS in a different storage buffer formulation.

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  IF Staining of BxPC-3 using 67498-1-Ig (same clone as 67498-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using AKR1B1 antibody (67498-1-Ig, Clone: 2F11G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67498-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67498-1-PBS cible AKR1B1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AKR1B1 Protéine recombinante Ag7571
• Nom complet: aldo-keto reductase family 1, member B1 (aldose reductase)
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC000260
• Symbole du gène: AKR1B1
• Identification du gène (NCBI): 231
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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