Anticorps Polyclonal de lapin anti-AKR1B10

AKR1B10 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (1)

N° de cat : 18252-1-AP

Synonymes

AKR1B10, AKR1B11, AKR1B12, Aldose reductase like, ALDRLn, ARL 1, ARL1, ARP, hARP, HSI, SI reductase, Small intestine reductase

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Galerie de données de validation

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  WB analysis using 18252-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18252-1-AP (AKR1B10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 18252-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18252-1-AP (AKR1B10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 18252-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18252-1-AP (AKR1B10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules A431, cellules HepG2, tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

18252-1-AP cible AKR1B10 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AKR1B10 Protéine recombinante Ag13042
• Nom complet: aldo-keto reductase family 1, member B10 (aldose reductase)
• Masse moléculaire calculée: 316 aa, 36 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC008837
• Symbole du gène: AKR1B10
• Identification du gène (NCBI): 57016
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	J Pharmacol Exp Ther; Mechanistic Analysis of an Extracellular Signal-Regulated Kinase 2-Interacting Compound that Inhibits Mutant BRAF-Expressing Melanoma Cells by Inducing Oxidative Stress.; Authors - Ramon Martinez

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shinford (Verified Customer) (10-26-2023)

In tissues, we detected bands at different positions, but in cancer cell lines, the bands appeared notably clearer. The optimal concentration was determined to be higher, specifically at 1:1000.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cancer cell lines and tumor tissues

AKR1B10 Antibody Western Blot validation (1:1000 dilution) in Cancer cell lines and tumor tissues (Cat no:18252-1-AP)