Anticorps Polyclonal de lapin anti-AKR1B10

AKR1B10 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

Avis (1)

N° de cat : 18252-1-AP

Synonymes

AKR1B 10, AKR1B11, AKR1B12, Aldo-keto reductase family 1 member B10, Aldose reductase-like

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Galerie de données de validation

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  WB analysis using 18252-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18252-1-AP (AKR1B10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 18252-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18252-1-AP (AKR1B10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 18252-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18252-1-AP (AKR1B10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 18252-1-AP

  IP result of anti-AKR1B10 (IP:18252-1-AP, 4ug; Detection:18252-1-AP 1:4000) with HepG2 cells lysate 2000 ug.

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  IHC staining of mouse stomach using 18252-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 18252-1-AP (AKR1B10 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 18252-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 18252-1-AP (AKR1B10 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules A431, cellules HepG2, tissu hépatique de souris
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu d'estomac de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

18252-1-AP cible AKR1B10 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AKR1B10 Protéine recombinante Ag13042
• Nom complet: aldo-keto reductase family 1, member B10 (aldose reductase)
• Masse moléculaire calculée: 316 aa, 36 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC008837
• Symbole du gène: AKR1B10
• Identification du gène (NCBI): 57016
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Aldo-keto reductase family 1 member B10 (AKR1B10) is a member of the AKR1B subfamily. It is mainly found in cytoplasm, and it is typically expressed in the stomach and intestines. Given that its expression is low or absent in other tissues, AKR1B10 is a potential diagnostic and therapeutic biomarker for various digestive system diseases.

Publications

Species	Application	Title
human	WB	J Pharmacol Exp Ther; Mechanistic Analysis of an Extracellular Signal-Regulated Kinase 2-Interacting Compound that Inhibits Mutant BRAF-Expressing Melanoma Cells by Inducing Oxidative Stress.; Authors - Ramon Martinez
mouse,human	WB	J Ethnopharmacol; Berberine directly targets AKR1B10 protein to modulate lipid and glucose metabolism disorders in NAFLD; Authors - Sa Yang ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shinford (Verified Customer) (10-26-2023)

In tissues, we detected bands at different positions, but in cancer cell lines, the bands appeared notably clearer. The optimal concentration was determined to be higher, specifically at 1:1000.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cancer cell lines and tumor tissues

AKR1B10 Antibody Western Blot validation (1:1000 dilution) in Cancer cell lines and tumor tissues (Cat no:18252-1-AP)

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