Anticorps Polyclonal de lapin anti-AKR1C3

AKR1C3 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(12)

N° de cat : 11194-1-AP

Synonymes

17-beta-HSD 5, 17-beta-hydroxysteroid dehydrogenase type 5, 3-alpha-HSD type 2, 3-alpha-HSD type II, brain, 3-alpha-hydroxysteroid dehydrogenase type 2

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Galerie de données de validation

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  WB analysis using 11194-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11194-1-AP (AKR1C3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 11194-1-AP

  IP result of anti-AKR1C3 (IP:11194-1-AP, 4ug; Detection:11194-1-AP 1:1000) with HepG2 cells lysate 2400ug.

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  IHC staining of human prostate cancer using 11194-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11194-1-AP (AKR1C3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 11194-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11194-1-AP (AKR1C3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HepG2, cellules Jurkat, cellules K-562
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IHC: See 4 publications below

Informations sur le produit

11194-1-AP cible AKR1C3 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AKR1C3 Protéine recombinante Ag1674
• Nom complet: aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II)
• Masse moléculaire calculée: 323 aa, 37 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC019230
• Symbole du gène: AKR1C3
• Identification du gène (NCBI): 8644
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AKR1C3(Aldo-keto reductase family 1 member C3) is also named as DDH1, HSD17B5, KIAA0119, PGFS and belongs to AKR1C family. .In humans, at least four AKR1C isoforms exist: AKR1C1, AKR1C2, AKR1C3, AKR1C4 and AKR1C3 shares >86% sequence identity with these three highly related human AKRs(PMID:18574251). It catalyzes the conversion of aldehydes and ketones to alcohols and androgen, estrogen, PG, xenobiotics metabolism. The rat kidney possesses a dimeric form of 75 kDa(PMID:18574251).

Publications

Species	Application	Title
human	WB	Mol Cancer Ther; AKR1C3 promotes AR-V7 protein stabilization and confers resistance to AR-targeted therapies in advanced prostate cancer.; Authors - Chengfei Liu
human	WB,IHC	World J Gastroenterol; Prostaglandin F2α synthase promotes oxaliplatin resistance in colorectal cancer through prostaglandin F2α-dependent and F2α-independent mechanism; Authors - Yi-Jun Wang
human	WB	Int J Mol Med; 15-Deoxy-Δ(12,14)-prostaglandin J(2) induces growth inhibition, cell cycle arrest and apoptosis in human endometrial cancer cell lines.; Authors - Li Haili H
rat,mouse	WB	Neurosci Lett; Mangiferin alleviates 6-OHDA-induced Parkinson's disease by inhibiting AKR1C3 to activate Wnt signaling pathway; Authors - Wanran Huang
human	IHC	J Clin Med; A New Histology-Based Prognostic Index for Aggressive T-Cell lymphoma: Preliminary Results of the "TCL Urayasu Classification"; Authors - Hideaki Nitta
human	WB	Transl Oncol; AKR1C3 promotes progression and mediates therapeutic resistance by inducing epithelial-mesenchymal transition and angiogenesis in small cell lung cancer; Authors - Wenting Liu

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