Anticorps Recombinant de lapin anti-AKR1C3

AKR1C3 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242222A11

N° de cat : 84872-5-PBS

Synonymes

17-beta-HSD 5, 17-beta-hydroxysteroid dehydrogenase type 5, 242222A11, 3-alpha-HSD type 2, 3-alpha-HSD type II, brain

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Galerie de données de validation

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  WB analysis using 84872-5-RR (same clone as 84872-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84872-5-RR (AKR1C3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84872-5-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 84872-5-RR (same clone as 84872-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84872-5-RR (AKR1C3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84872-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84872-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84872-5-RR against Human AKR1C3 were performed. The affinity constant is 35.8 nM.

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Informations sur le produit

84872-5-PBS cible AKR1C3 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: AKR1C3 Protéine recombinante Ag1674
• Nom complet: aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II)
• Masse moléculaire calculée: 323 aa, 37 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC019230
• Symbole du gène: AKR1C3
• Identification du gène (NCBI): 8644
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AKR1C3(Aldo-keto reductase family 1 member C3) is also named as DDH1, HSD17B5, KIAA0119, PGFS and belongs to AKR1C family. .In humans, at least four AKR1C isoforms exist: AKR1C1, AKR1C2, AKR1C3, AKR1C4 and AKR1C3 shares >86% sequence identity with these three highly related human AKRs(PMID:18574251). It catalyzes the conversion of aldehydes and ketones to alcohols and androgen, estrogen, PG, xenobiotics metabolism.

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