Anticorps Polyclonal de lapin anti-AKR7A2

• Phare
• Validé par KD/KO

AKR7A2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 10730-1-AP

Synonymes

AFAR, AFAR1, AFB1 aldehyde reductase 1, AFB1 AR 1, AFB1 AR1, AKR7, AKR7A2, Aldoketoreductase 7, SSA reductase

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Galerie de données de validation

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  WB analysis of HEK-293 using 10730-1-AP

  WB result of AKR7A2 antibody (10730-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKR7A2 transfected HEK-293 cells.

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  WB analysis of mouse liver using 10730-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10730-1-AP (AKR7A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10730-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10730-1-AP (AKR7A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10730-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10730-1-AP (AKR7A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 10730-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10730-1-AP (AKR7A2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10730-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10730-1-AP (AKR7A2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules A431, cellules HEK-293, cellules HepG2
• Résultats positifs en IHC: tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

10730-1-AP cible AKR7A2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AKR7A2 Protéine recombinante Ag1049
• Nom complet: aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase)
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC007352
• Symbole du gène: AKR7A2
• Identification du gène (NCBI): 8574
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AKR7A2(Aflatoxin B1 aldehyde reductase member 2) is also named as AFAR, AFAR1, AKR7 and belongs to the aldo/keto reductase family. AKR7A2 enzyme is catalytically active toward aldehydes arising from lipid peroxidation, suggesting a potential role against the consequences of oxidative stress, and representing an important detoxication route in mammalian cells (PMID:22001351).

Publications

Species	Application	Title
mouse	WB	Environ Toxicol; Naringin alleviates acetaminophen-induced acute liver injury by activating Nrf2 via CHAC2 upregulation.; Authors - Xiaohan Zhai
human	WB,IHC	Pancreatology; Proteomic analysis of pancreatic ductal adenocarcinoma compared with normal adjacent pancreatic tissue and pancreatic benign cystadenoma.; Authors - Cui Yazhou Y

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