Anticorps Polyclonal de lapin anti-AKT

• Phare
• Validé par KD/KO

AKT Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (6)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

1060

Avis (2)

N° de cat : 10176-2-AP

Synonymes

AKT, AKT1, AKT2, AKT3, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha

---

Galerie de données de validation

• 

  WB analysis of MCF-7 using 10176-2-AP

  WB result of AKT antibody (10176-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT transfected MCF-7 cells.

• 

  WB analysis of HeLa cells using 10176-2-AP

  siRNA AKT1 result from Dr. Eva Martinez-Balibrea. Green:tubulin, Red:10176-2-AP, AKT1.

• 

  WB analysis using 10176-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10176-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10176-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10176-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10176-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT1 antibody) at dilution of 1:500.

• 

  IP experiment of HeLa using 10176-2-AP

  IP Result of anti-AKT1 (IP:10176-2-AP, 3ug; Detection:10176-2-AP 1:500) with HeLa cells lysate 2500ug.

• 

  IHC staining of human ovary tumor using 10176-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10176-2-AP (AKT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10176-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10176-2-AP (AKT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10176-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10176-2-AP (AKT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10176-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10176-2-AP (AKT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 10176-2-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using AKT antibody (10176-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10176-2-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 10176-2-AP (AKT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 10176-2-AP

  1X10^6 HeLa cells were stained with 0.2ug AKT1 antibody (10176-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules C6, cellules HeLa, cellules HepG2, cellules MCF-7, cellules NIH/3T3, tissu cérébral de rat, tissu cérébral de souris, tissu hépatique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, tissu cérébral de souris
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 1034 publications below
• IHC: See 31 publications below
• IF: See 16 publications below
• IP: See 6 publications below
• ELISA: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

10176-2-AP cible AKT dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, poisson-zèbre, porc, poulet, souris, Hamster, fish
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AKT Protéine recombinante Ag0213
• Nom complet: v-akt murine thymoma viral oncogene homolog 1
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 56-62 kDa
• Numéro d’acquisition GenBank: BC000479
• Symbole du gène: AKT1
• Identification du gène (NCBI): 207
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

Publications

Species	Application	Title
human	WB	Cell Res; RAB31 marks and controls an ESCRT-independent exosome pathway.; Authors - Denghui Wei
mouse	WB	Nat Cell Biol; Heat stress activates YAP/TAZ to induce the heat shock transcriptome.; Authors - Min Luo
human	WB	Nat Commun; Genome-wide enhancer-gene regulatory maps link causal variants to target genes underlying human cancer risk; Authors - Pingting Ying
human	WB	Sci Adv; Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes.; Authors - Patrick Han
mouse	WB	Acta Pharm Sin B; Oral nano-formulation improves pancreatic islets dysfunction via lymphatic transport for antidiabetic treatment; Authors - Lin Hou
human	WB	Brain; MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation.; Authors - Bing-Lin Zhu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Hyopil (Verified Customer) (08-23-2021)

Worked well with western blotting

• Applications: Western Blot
• Cell Tissue Type: HAP1

FH

Azita (Verified Customer) (06-02-2021)

Western blot analysis using AKT polyclonal antibody in NSC34 cell line at dilution of 1:500.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: NSC34

Produits associés

mTOR Polyclonal antibody

20657-1-AP

PI3 Kinase p110 Alpha Polyclonal antibody

20583-1-AP

PI3 Kinase p110 Beta Polyclonal antibody

20584-1-AP

PDK1 Polyclonal antibody

18262-1-AP

GSK3B Polyclonal antibody

22104-1-AP