Anticorps Monoclonal anti-AKT

• Phare
• Validé par KD/KO

AKT Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris et plus (6)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(637)

CloneNo.

2C5D1

Avis (3)

N° de cat : 60203-2-Ig

Synonymes

AKT 1, AKT1, 2C5D1, EC:2.7.11.1, PKB

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Galerie de données de validation

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  WB analysis using 60203-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 60203-2-Ig

  WB result of AKT antibody (60203-2-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT transfected A549 cells.

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  WB analysis of HEK-293 using 60203-2-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) and 66444-1-Ig(AKT1-phospho-S473 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 60203-2-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 60203-2-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60203-2-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 60203-2-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 60203-2-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 60203-2-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 60203-2-Ig

  IP result of anti-AKT (IP:60203-2-Ig, 5ug; Detection:60203-2-Ig 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human breast cancer using 60203-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-2-Ig (AKT Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 60203-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-2-Ig (AKT Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 60203-2-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 60203-2-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 60203-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 60203-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 60203-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 60203-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 60203-2-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using AKT antibody (60203-2-Ig, Clone: 2C5D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules LNCaP, cellules NIH/3T3, cellules PC-12, cellules RAW 264.7, cellules ROS1728, tissu cérébral de souris, tissu hépatique de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 612 publications below
• IHC: See 17 publications below
• IF: See 15 publications below
• IP: See 3 publications below
• CoIP: See 4 publications below

Informations sur le produit

60203-2-Ig cible AKT dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Chèvre, Humain, Lapin, poulet, souris, Hamster, duck
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AKT Protéine recombinante Ag16695
• Nom complet: v-akt murine thymoma viral oncogene homolog 1
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 56-62 kDa
• Numéro d’acquisition GenBank: BC000479
• Symbole du gène: AKT1
• Identification du gène (NCBI): 207
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with/without phospho- modification.

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther; Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis; Authors - Chuan Yang
mouse	WB	Cell Metab; Acetate enables metabolic fitness and cognitive performance during sleep disruption; Authors - Qinqin He
mouse	WB	Nat Commun; FOXP3+ regulatory T cell perturbation mediated by the IFNγ-STAT1-IFITM3 feedback loop is essential for anti-tumor immunity; Authors - Xinnan Liu
human	WB	Cell Rep Med; Reprograming immunosuppressive microenvironment by eIF4G1 targeting to eradicate pancreatic ductal adenocarcinoma; Authors - Lihong He
mouse	WB	Cell Rep Med; Targeting phenylpyruvate restrains excessive NLRP3 inflammasome activation and pathological inflammation in diabetic wound healing; Authors - Dongming Lv
human	WB	Adv Healthc Mater; A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction.; Authors - Zhi Zheng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

A (Verified Customer) (07-15-2025)

Used for caco2 cells and animal colon tissue

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1 to 1000

FH

C (Verified Customer) (06-25-2025)

A reliable Ab to detect endo Akt.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain lysate

AKT Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:60203-2-Ig)

FH

Janine (Verified Customer) (02-18-2019)

Yielded clear and distinguishable bands at the expected size of ~60kDa after 10 sec exposure using enhanced chemiluminescence.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: RPE-1 cell lysates (6cm dish)

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