Anticorps Monoclonal anti-AKT

• Phare
• Validé par KD/KO

AKT Monoclonal Antibody for WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2C5D1

N° de cat : 60203-2-PBS

Synonymes

AKT 1, AKT1, 2C5D1, EC:2.7.11.1, PKB

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Galerie de données de validation

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  WB analysis using 60203-2-Ig (same clone as 60203-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 60203-2-Ig (same clone as 60203-2-PBS)

  WB result of AKT antibody (60203-2-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT transfected A549 cells. This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 60203-2-Ig (same clone as 60203-2-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) and 66444-1-Ig(AKT1-phospho-S473 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 60203-2-Ig (same clone as 60203-2-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 60203-2-Ig (same clone as 60203-2-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 60203-2-Ig (same clone as 60203-2-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 60203-2-Ig (same clone as 60203-2-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 60203-2-Ig (same clone as 60203-2-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

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  WB analysis of ROS1728 using 60203-2-Ig (same clone as 60203-2-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 60203-2-Ig (same clone as 60203-2-PBS)

  IP result of anti-AKT (IP:60203-2-Ig, 5ug; Detection:60203-2-Ig 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60203-2-Ig (same clone as 60203-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-2-Ig (AKT Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60203-2-Ig (same clone as 60203-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-2-Ig (AKT Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 60203-2-Ig (same clone as 60203-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 60203-2-Ig (same clone as 60203-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60203-2-Ig (same clone as 60203-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60203-2-Ig (same clone as 60203-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60203-2-Ig (same clone as 60203-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60203-2-Ig (same clone as 60203-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 60203-2-Ig (same clone as 60203-2-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using AKT antibody (60203-2-Ig, Clone: 2C5D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

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  Cytometric bead array in cell lysate using MP50177-1

  Cytometric bead array in cell lysate using MP50177-1, Phospho-AKT(Ser473) Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66444-1-PBS. Detection antibody: 60203-2-PBS. Cell lysate: Non-treated HEK293 and Calyculin A treated HEK293(30μg/well). Non-related target OAT Monoclonal Matched Antibody Pair (MP50109-1P) was served as control.

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Informations sur le produit

60203-2-PBS cible AKT dans les applications de WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AKT Protéine recombinante Ag16695
• Nom complet: v-akt murine thymoma viral oncogene homolog 1
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 56-62 kDa
• Numéro d’acquisition GenBank: BC000479
• Symbole du gène: AKT1
• Identification du gène (NCBI): 207
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with/without phospho- modification.

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