Anticorps Recombinant de lapin anti-AKT1-Specific

• Phare
• Validé par KD/KO

AKT1-Specific Recombinant Antibody for WB, IHC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

5O9

N° de cat : 80816-1-RR

Synonymes

AKT, AKT 1, AKT1, 5O9, EC:2.7.11.1

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Galerie de données de validation

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  WB analysis using 80816-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80816-1-RR (AKT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 80816-1-RR

  WB result of AKT1 antibody (80816-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT1 transfected MCF-7 cells.

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  IP experiment of HEK-293 using 80816-1-RR

  IP result of anti-AKT1 (IP:80816-1-RR, 4ug; Detection:80816-1-RR 1:2000) with HEK-293 cells lysate 1280 ug.

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  IHC staining of human ovary tumor using 80816-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80816-1-RR (AKT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 80816-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80816-1-RR (AKT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of Jurkat using 80816-1-RR

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human AKT1 (80816-1-RR, Clone:5O9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug control antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A549, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules MCF-7, cellules NIH/3T3, cellules PC-12, cellules RAW 264.7
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

80816-1-RR cible AKT1-Specific dans les applications de WB, IHC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: AKT1-Specific Protéine recombinante Ag0213
• Nom complet: v-akt murine thymoma viral oncogene homolog 1
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 56-62 kDa
• Numéro d’acquisition GenBank: BC000479
• Symbole du gène: AKT1
• Identification du gène (NCBI): 207
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. 80816-1-RR recognizes AKT1 specifically.

Publications

Species	Application	Title
mouse,human	WB	Int Immunopharmacol; Geniposide ameliorates psoriatic skin inflammation by inhibiting the TLR4/MyD88/NF-κB p65 signaling pathway and MMP9; Authors - Lijuan Liu
mouse	WB	Int J Mol Sci; Rotenone Induces Parkinsonism with Constipation Symptoms in Mice by Disrupting the Gut Microecosystem, Inhibiting the PI3K-AKT Signaling Pathway and Gastrointestinal Motility; Authors - Li Liu
mouse	WB	Int J Biol Macromol; An inulin-type polysaccharide from Atractylodis Macrocephalae Rhizoma can relieve psoriasis; Authors - Xinran Tang
mouse	IHC	Int J Biol Macromol; Water-insoluble dietary fiber from walnut relieves constipation through Limosilactobacillus reuteri-mediated serotonergic synapse and neuroactive ligand-receptor pathways; Authors - Weixing Yang
mouse	WB	Mol Med; Lycorine ameliorates liver steatosis, oxidative stress, ferroptosis and intestinal homeostasis imbalance in MASLD mice; Authors - Ziwen Wang
human	WB	Adv Sci (Weinh); Injectable pH Responsive Conductive Hydrogel for Intelligent Delivery of Metformin and Exosomes to Enhance Cardiac Repair after Myocardial Ischemia-Reperfusion Injury; Authors - Nianlan Cheng

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