Anticorps Polyclonal de lapin anti-AKT2

AKT2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 28113-1-AP

Synonymes

PKBBETA, PKBB, PKB beta, EC:2.7.11.1, AKT2 protein

---

Galerie de données de validation

• 

  WB analysis using 28113-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28113-1-AP (AKT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 28113-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28113-1-AP (AKT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 28113-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28113-1-AP (AKT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of NIH/3T3 using 28113-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using AKT2 antibody (28113-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of NIH/3T3 using 28113-1-AP

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Human AKT2 (28113-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules NIH/3T3, tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules NIH/3T3,
• Résultats positifs en FC (Intra): cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

28113-1-AP cible AKT2 dans les applications de WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AKT2 Protéine recombinante Ag27005
• Nom complet: v-akt murine thymoma viral oncogene homolog 2
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC120994
• Symbole du gène: AKT2
• Identification du gène (NCBI): 208
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis and their activation has been observed in a wide variety of cancers. AKT2 is mainly involved in cancer cell survival, apoptosis inhibition, migration and invasion(PMID:21979951). Defects in AKT2 are a cause of susceptibility to breast cancer (BC). AKT2 promotes metastasis of tumor cells without affecting the latency of tumor development. And defects in AKT2 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) and hypoinsulinemic hypoglycemia with hemihypertrophy (HIHGHH). The full length protein has four glycosylation sites.

Publications

Species	Application	Title
human	WB	Int J Biochem Cell Biol; AKT serine/threonine kinase 2-mediated phosphorylation of fascin threonine 403 regulates esophageal cancer progression.; Authors - Zhi-Da Zhang
mouse	WB,CoIP	Biologics; ATF3 Knockdown Exacerbates Astrocyte Activation by Inhibiting Phosphorylation of Drp1 in Ischemic Stroke; Authors - Rong Huang
mouse	WB	Chem Res Toxicol; Novel Dual PPAR δ/γ Partial Agonist Induces Hepatic Lipid Accumulation through Direct Binding and Inhibition of AKT1 Phosphorylation, Mediating CD36 Upregulation; Authors - Xiaotong Cai
human	IHC	Oncogene; The protection of UCK2 protein stability by GART maintains pyrimidine salvage synthesis for HCC growth under glucose limitation; Authors - Nannan Sha

{{ptg:RelatedPrimaryAntibodies}}