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Anticorps Polyclonal de lapin anti-ALAS2

ALAS2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30539-1-PBS

Synonymes

ALAS-E, ALASE, ALAS E, ALAS 2, 5-aminolevulinic acid synthase 2

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using ALAS2 Polyclonal antibody (30539-1-AP)

    WB analysis using 30539-1-AP (same clone as 30539-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30539-1-AP (ALAS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30539-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ALAS2 Polyclonal antibody (30539-1-AP)

    IF Staining of HEK-293 using 30539-1-AP (same clone as 30539-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ALAS2 antibody (30539-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 30539-1-PBS in a different storage buffer formulation.

Informations sur le produit

30539-1-PBS cible ALAS2 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène ALAS2 Protéine recombinante Ag30308
Nom complet aminolevulinate, delta-, synthase 2
Masse moléculaire calculée 587 aa, 65 kDa
Poids moléculaire observé65 kDa
Numéro d’acquisition GenBankBC030230
Symbole du gène ALAS2
Identification du gène (NCBI) 212
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The heme biosynthetic pathway begins from delta aminolevulinic acid synthase (ALAS) catalyzing the condensation of glycine and succinyl-CoA to delta aminolevulinic acid (ALA) in the mitochondria. ALAS is coded by two genes: ALAS1 and ALAS2 . ALAS1 is ubiquitously expressed in all cells, and the negative feedback is regulated by the heme pool; however, ALAS2 is specifically expressed only in erythroid cells.

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