Anticorps Monoclonal anti-ALAS2

ALAS2 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2C1H1

N° de cat : 68332-1-PBS

Synonymes

ALASE, ALAS E, ALAS 2, 5-aminolevulinic acid synthase 2, 5-aminolevulinate synthase, erythroid-specific, mitochondrial

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Galerie de données de validation

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  WB analysis using 68332-1-Ig (same clone as 68332-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68332-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68332-1-Ig (same clone as 68332-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68332-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68332-1-Ig (same clone as 68332-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68332-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NK-92 using 68332-1-Ig (same clone as 68332-1-PBS)

  NK-92 cells were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68332-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50624-1

  Cytometric bead array standard curve of MP50624-1, ALAS2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68332-1-PBS. Detection antibody: 68332-2-PBS. Standard:Ag13524. Range: 6.25-100 ng/mL.

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  Cytometric bead array standard curve of MP50624-2

  Cytometric bead array standard curve of MP50624-2, ALAS2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68332-1-PBS. Detection antibody: 68332-3-PBS. Standard:Ag13524. Range: 3.125-100 ng/mL.

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Informations sur le produit

68332-1-PBS cible ALAS2 dans les applications de WB, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ALAS2 Protéine recombinante Ag13524
• Nom complet: aminolevulinate, delta-, synthase 2
• Masse moléculaire calculée: 587 aa, 65 kDa
• Poids moléculaire observé: 60~65 kDa
• Numéro d’acquisition GenBank: BC030230
• Symbole du gène: ALAS2
• Identification du gène (NCBI): 212
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The heme biosynthetic pathway begins from delta aminolevulinic acid synthase (ALAS) catalyzing the condensation of glycine and succinyl-CoA to delta aminolevulinic acid (ALA) in the mitochondria. ALAS is coded by two genes: ALAS1 and ALAS2 . ALAS1 is ubiquitously expressed in all cells, and the negative feedback is regulated by the heme pool; however, ALAS2 is specifically expressed only in erythroid cells.

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