Anticorps Polyclonal de lapin anti-Albumin

• Phare
• Validé par KD/KO

Albumin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(187)

Avis (2)

N° de cat : 16475-1-AP

Synonymes

ALB, Serum albumin, HSA

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• Galerie de données de validation
• Proteintech vs. Other Vendors

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  WB analysis of HepG2 using 16475-1-AP

  WB result of Albumin antibody (16475-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Albumin transfected HepG2 cells.

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  WB analysis of human plasma using 16475-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 16475-1-AP (ALB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16475-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16475-1-AP (Albumin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse serum using 16475-1-AP

  mouse serum were subjected to SDS PAGE followed by western blot with 16475-1-AP (Albumin Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of human plasma using 16475-1-AP

  IP result of anti-Albumin (IP:16475-1-AP, 4ug; Detection:16475-1-AP 1:10000) with human plasma lysate 4000ug.

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  IHC staining of human liver using 16475-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16475-1-AP (Albumin antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 16475-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16475-1-AP (Albumin antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 16475-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 16475-1-AP (Albumin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 16475-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16475-1-AP (Albumin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 16475-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16475-1-AP (Albumin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 16475-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (16475-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 16475-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (16475-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 16475-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Albumin antibody (16475-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of L02 using 16475-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using Albumin antibody (16475-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu sanguin humain, cellules HepG2, plasma humain, sérum de souris, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: tissu plasmatique humain
• Résultats positifs en IHC: tissu hépatique humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu hépatique de souris,
• Résultats positifs en IF/ICC: cellules L02,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 107 publications below
• IHC: See 17 publications below
• IF: See 73 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

16475-1-AP cible Albumin dans les applications de WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Albumin Protéine recombinante Ag9569
• Nom complet: albumin
• Masse moléculaire calculée: 609 aa, 69 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC034023
• Symbole du gène: Albumin
• Identification du gène (NCBI): 213
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. In additon, glycated serum albumin is also a potential diabetes biomarker. The N-terminal 24aa of albumin will be cleaved to generate the mature form of serum albumin with MW 66 kDa. (21388516, 23832071)

Publications

Species	Application	Title
human,mouse	WB	Science; Enterically derived high-density lipoprotein restrains liver injury through the portal vein.; Authors - Yong-Hyun Han
mouse	IF	Signal Transduct Target Ther; MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration; Authors - Jinpeng Du
human,mouse	WB,IF	Signal Transduct Target Ther; The AKAP12-PKA axis regulates lipid homeostasis during alcohol-associated liver disease; Authors - Chandana Thimme Gowda
human, mouse	WB,IF	Nat Immunol; Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.; Authors - Li Jianhua J
human	IHC	Immunity; The zinc finger protein Miz1 suppresses liver tumorigenesis by restricting hepatocyte-driven macrophage activation and inflammation.; Authors - Wenjie Zhang
mouse	WB	Nat Cell Biol; Manganese regulation of COPII condensation controls circulating lipid homeostasis; Authors - Xiao Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kamal (Verified Customer) (01-26-2024)

For western blot analysis, antibody worked well at dilution of 1:25000 in 1 X TBST. For immunofluorescence, antibody worked well at dilution of 1:200 in 1 X PBS.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Mouse bone marrow

FH

Kamal (Verified Customer) (01-26-2024)

Liver tissues were well stained in provided dilution with 1X PBS.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse liver tissue

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