Anticorps Recombinant de lapin anti-ALCAM

ALCAM Recombinant Antibody for IF/ICC, FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

IF/ICC, FC

Conjugaison

Non conjugué

CloneNo.

3A6

N° de cat : 65611-1-MR

Synonymes

MEMD, CD166, 3A6

---

Galerie de données de validation

• 

  IF Staining of hTERT-RPE1 using 65611-1-MR

  Immunofluorescent analysis of un-fixed hTERT-RPE1 cells using ALCAM antibody (65611-1-MR, Clone: 3A6 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

• 

  FC experiment of human PBMCs using 65611-1-MR

  1x10^6 PHA treated human PBMCs were surface stained with 0.25 ug Anti-Human ALCAM Mouse IgG2a Recombinant Antibody (65611-1-MR, Clone: 3A6) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Untreated human PBMCs were surface stained with 0.25 ug Anti-Human ALCAM Mouse IgG2a Recombinant Antibody (65611-1-MR, Clone: 3A6) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) (black). Cells were not fixed.

---

Applications testées

• Résultats positifs en IF/ICC: cellules hTERT-RPE1,
• Résultats positifs en cytométrie: PHA treated human PBMCs,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

65611-1-MR cible ALCAM dans les applications de IF/ICC, FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cultured human thymic epithelial cells
• Nom complet: activated leukocyte cell adhesion molecule
• Masse moléculaire calculée: 105 kDa
• Numéro d’acquisition GenBank: BC057809
• Symbole du gène: ALCAM
• Identification du gène (NCBI): 214
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues and cell types, including activated T cells and monocytes, epithelial cells, fibroblasts, neuronal cells, hepatocytes, and bone marrow mesenchymal stem cells (PMID: 7760007; 25221999). Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).

{{ptg:RelatedPrimaryAntibodies}}