Anticorps Monoclonal anti-ALCAM

ALCAM Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F7D6

N° de cat : 67768-1-PBS

Synonymes

1F7D6, CD166, MEMD

---

Galerie de données de validation

• 

  WB analysis using 67768-1-Ig (same clone as 67768-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67768-1-Ig (ALCAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67768-1-Ig (ALCAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67768-1-Ig (ALCAM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

  Immunofluorescent analysis of un-fixed frozen OCT-embedded mouse breast cancer using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

• 

  IF Staining of hTERT-RPE1 using 67768-1-Ig (same clone as 67768-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

67768-1-PBS cible ALCAM dans les applications de WB, IHC, IF/ICC, IF-P, IF-Fro, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ALCAM Protéine recombinante Ag16892
• Nom complet: activated leukocyte cell adhesion molecule
• Masse moléculaire calculée: 583 aa, 65 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: BC137097
• Symbole du gène: ALCAM
• Identification du gène (NCBI): 214
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).

{{ptg:RelatedPrimaryAntibodies}}