Anticorps Recombinant de lapin anti-ALCAM

ALCAM Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

241945A4

N° de cat : 84528-1-PBS

Synonymes

241945A4, Activated leukocyte cell adhesion molecule, BEN, CD166, CD166 antigen

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Galerie de données de validation

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  WB analysis of mouse lung using 84528-1-RR (same clone as 84528-1-PBS)

  mouse lung tissue was subjected to SDS PAGE followed by western blot with 84528-1-RR (ALCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84528-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01379-1

  Sandwich ELISA standard curve of MP01379-1, Mouse ALCAM Recombinant Matched Antibody Pair - PBS only. 84528-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1524. 84528-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

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  Sample test of MP01379-1

  Serum of six mice was measured. The ALCAM concentration of detected samples was determined to be 43,380.5 pg/mL with a range of 28,091.3-50,380.4 pg/mL.

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  Sample test of MP01379-1

  The mean ALCAM concentration was determined to be 888,916.9 pg/mL in mouse liver tissue extract based on a 3.7 mg/mL extract load and 518,424.5 pg/mL in mouse brain tissue extract based on a 2.0 mg/mL extract load.

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  IHC staining of mouse liver using 84528-1-RR (same clone as 84528-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84528-1-RR (ALCAM antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84528-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84528-1-RR (same clone as 84528-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84528-1-RR (ALCAM antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84528-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01379-1

  Cytometric bead array standard curve of MP01379-1, MOUSE ALCAM Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84528-1-PBS. Detection antibody: 84528-3-PBS. Standard: Eg1524. Range: 0.781-100 ng/mL.

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Informations sur le produit

84528-1-PBS cible ALCAM dans les applications de WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ALCAM Protéine recombinante Eg1524
• Nom complet: activated leukocyte cell adhesion molecule
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: NM_009655.3
• Symbole du gène: Alcam
• Identification du gène (NCBI): 11658
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).

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