Anticorps Recombinant de lapin anti-ALDH1A2

ALDH1A2 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251371D10

N° de cat : 86518-1-PBS

Synonymes

ALDH1A 2, EC:1.2.1.36, RALDH 2, RALDH(II), RalDH2

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Galerie de données de validation

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  WB analysis using 86518-1-RR (same clone as 86518-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86518-1-RR (ALDH1A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86518-1-PBS in a different storage buffer formulation.

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  IF Staining of K-562 using 86518-1-RR (same clone as 86518-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using ALDH1A2 antibody (86518-1-RR, Clone: 251371D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86518-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86518-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86518-1-RR against Human ALDH1A2 were performed. The affinity constant is 0.179 nM.

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Informations sur le produit

86518-1-PBS cible ALDH1A2 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ALDH1A2 Protéine recombinante Ag5062
• Nom complet: aldehyde dehydrogenase 1 family, member A2
• Masse moléculaire calculée: 518 aa, 57 kDa
• Poids moléculaire observé: 53-57 kDa
• Numéro d’acquisition GenBank: BC030589
• Symbole du gène: ALDH1A2
• Identification du gène (NCBI): 8854
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ALDH1A2(aldehyde dehydrogenase family 1 member A2) is also named as RALDH2(retinal dehydrogenase 2) and belongs to the aldehyde dehydrogenase family. It is a cytosolic homotetramer(56.7 kDa subunits) exhibiting complex expression patterns throughout embryonic development. ALDH1A2 plays a role in retinoid metabolism during embryonic development where it is considered to be the major retinoic acid-synthesizing enzyme during early embryogenesis(PMID:20735668). Its expression is reduced in primary prostate tumors compared with normal prostate tissue suggested that it is normally expressed in the epithelial compartment of prostate tissue(PMID:16166285).It has 2 isoforms produced by alternative splicing.

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