Anticorps Recombinant de lapin anti-ALDH1A2

ALDH1A2 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

251371D10

N° de cat : 86518-1-RR

Synonymes

ALDH1A 2, EC:1.2.1.36, RALDH 2, RALDH(II), RalDH2

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Galerie de données de validation

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  WB analysis using 86518-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86518-1-RR (ALDH1A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of K-562 using 86518-1-RR

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using ALDH1A2 antibody (86518-1-RR, Clone: 251371D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86518-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86518-1-RR against Human ALDH1A2 were performed. The affinity constant is 0.179 nM.

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Applications testées

• Résultats positifs en WB: tissu splénique de souris, testicule humain, tissu splénique de rat, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IF/ICC: cellules K-562,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86518-1-RR cible ALDH1A2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ALDH1A2 Protéine recombinante Ag5062
• Nom complet: aldehyde dehydrogenase 1 family, member A2
• Masse moléculaire calculée: 518 aa, 57 kDa
• Poids moléculaire observé: 53-57 kDa
• Numéro d’acquisition GenBank: BC030589
• Symbole du gène: ALDH1A2
• Identification du gène (NCBI): 8854
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ALDH1A2(aldehyde dehydrogenase family 1 member A2) is also named as RALDH2(retinal dehydrogenase 2) and belongs to the aldehyde dehydrogenase family. It is a cytosolic homotetramer(56.7 kDa subunits) exhibiting complex expression patterns throughout embryonic development. ALDH1A2 plays a role in retinoid metabolism during embryonic development where it is considered to be the major retinoic acid-synthesizing enzyme during early embryogenesis(PMID:20735668). Its expression is reduced in primary prostate tumors compared with normal prostate tissue suggested that it is normally expressed in the epithelial compartment of prostate tissue(PMID:16166285).It has 2 isoforms produced by alternative splicing.

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