Anticorps Polyclonal de lapin anti-ALDH1B1

ALDH1B1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 22220-1-PBS

Synonymes

Aldehyde dehydrogenase 5, Aldehyde dehydrogenase X, mitochondrial, ALDH5, ALDHX, EC:1.2.1.3

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Galerie de données de validation

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  WB analysis of HeLa using 22220-1-AP (same clone as 22220-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22220-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 22220-1-AP (same clone as 22220-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22220-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 22220-1-AP (same clone as 22220-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22220-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 22220-1-AP (same clone as 22220-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22220-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 22220-1-AP (same clone as 22220-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22220-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 22220-1-AP (same clone as 22220-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22220-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 22220-1-AP (same clone as 22220-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22220-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 22220-1-AP (same clone as 22220-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22220-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 22220-1-AP (same clone as 22220-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22220-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 22220-1-AP (same clone as 22220-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22220-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 22220-1-AP (same clone as 22220-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22220-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 22220-1-AP (same clone as 22220-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22220-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 22220-1-AP (same clone as 22220-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver slide using 22220-1-AP (ALDH1B1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 22220-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 22220-1-AP (same clone as 22220-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver slide using 22220-1-AP (ALDH1B1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 22220-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 22220-1-AP (same clone as 22220-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ALDH1B1 antibody (22220-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22220-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 22220-1-AP (same clone as 22220-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ALDH1B1 antibody (22220-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 22220-1-PBS in a different storage buffer formulation.

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Informations sur le produit

22220-1-PBS cible ALDH1B1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ALDH1B1 Protéine recombinante Ag17557
• Nom complet: aldehyde dehydrogenase 1 family, member B1
• Masse moléculaire calculée: 517 aa, 57 kDa
• Poids moléculaire observé: 55-58 kDa
• Numéro d’acquisition GenBank: BC001619
• Symbole du gène: ALDH1B1
• Identification du gène (NCBI): 219
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Aldehyde dehydrogenases (ALDHs) belong to a superfamily of NAD(P)+-dependent enzymes, which catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding acids. ALDH1B1 is a mitochondrial ALDH that it is dramatically upregulated in human colonic adenocarcinoma, making it a potential biomarker for human colon cancer.(PMID:21216231). ALDH1B1 is a homotetramer expressed in various adult and fetal human tissues including liver, testis, kidney, skeletal muscle, heart, placenta, brain and lung(PMID:18611112). This antibody is specific to ALDH1B1.

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