Anticorps Monoclonal anti-ALDH1L1

ALDH1L1 Monoclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF-P, IF-Fro, ELISA

Conjugaison

Non conjugué

CloneNo.

1C1A3

N° de cat : 68018-1-PBS

Synonymes

10 FTHFDH, 10-FTHFDH, 1C1A3, ALDH1L 1, Cytosolic 10-formyltetrahydrofolate dehydrogenase

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Galerie de données de validation

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  WB analysis of human milk using 68018-1-Ig (same clone as 68018-1-PBS)

  human milk were subjected to SDS PAGE followed by western blot with 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68018-1-PBS in a different storage buffer formulation.

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  WB analysis of pig liver using 68018-1-Ig (same clone as 68018-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68018-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 68018-1-Ig (same clone as 68018-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68018-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 68018-1-Ig (same clone as 68018-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68018-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68018-1-Ig (same clone as 68018-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68018-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68018-1-Ig (same clone as 68018-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:32000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68018-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 68018-1-Ig (same clone as 68018-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ALDH1L1 antibody (68018-1-Ig, Clone: 1C1A3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68018-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 68018-1-Ig (same clone as 68018-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using ALDH1L1 antibody (68018-1-Ig, Clone: 1C1A3 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-3). This data was developed using the same antibody clone with 68018-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68018-1-PBS cible ALDH1L1 dans les applications de WB, IHC, IF-P, IF-Fro, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ALDH1L1 Protéine recombinante Ag13190
• Nom complet: aldehyde dehydrogenase 1 family, member L1
• Masse moléculaire calculée: 99 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC027241
• Symbole du gène: ALDH1L1
• Identification du gène (NCBI): 10840
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Aldehyde dehydrogenase 1 family member L1 (ALDH1L1), belongs to the aldehyde dehydrogenase family of proteins and is responsible for formate oxidation in vivo. Loss of ALDH1L1 is associated with decreased apoptosis, increased cell motility, and cancer progression. ALDH1L1 catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. ALDH1L1 has some isoforms with MW of 99, 88 and 55 kDa.

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