Anticorps Recombinant de lapin anti-ALDH1L1

ALDH1L1 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250565B12

N° de cat : 85980-2-RR

Synonymes

10 FTHFDH, 10-FTHFDH, ALDH1L 1, Cytosolic 10-formyltetrahydrofolate dehydrogenase, EC:1.5.1.6

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Galerie de données de validation

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  WB analysis using 85980-2-RR

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 85980-2-RR (ALDH1L1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Neuro-2a using 85980-2-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using ALDH1L1 antibody (85980-2-RR, Clone: 250565B12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 85980-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85980-2-RR against Human ALDH1L1 were performed. The affinity constant is 2.04 nM.

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu hépatique de rat
• Résultats positifs en IF/ICC: cellules Neuro-2a,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85980-2-RR cible ALDH1L1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ALDH1L1 Protéine recombinante Ag11285
• Nom complet: aldehyde dehydrogenase 1 family, member L1
• Masse moléculaire calculée: 99 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC027241
• Symbole du gène: ALDH1L1
• Identification du gène (NCBI): 10840
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Aldehyde dehydrogenase 1 family member L1 (ALDH1L1), belongs to the aldehyde dehydrogenase family of proteins and is responsible for formate oxidation in vivo. Loss of ALDH1L1 is associated with decreased apoptosis, increased cell motility, and cancer progression. ALDH1L1 catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. ALDH1L1 has some isoforms with MW of 99, 88 and 55 kDa.

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