Anticorps Recombinant de lapin anti-ALDH2

ALDH2 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251287E9

N° de cat : 86429-5-PBS

Synonymes

ALDH 2, ALDH class 2, ALDH E2, ALDHE2, ALDH-E2

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Galerie de données de validation

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  WB analysis using 86429-5-RR (same clone as 86429-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86429-5-RR (ALDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86429-5-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 86429-5-RR (same clone as 86429-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using ALDH2 antibody (86429-5-RR, Clone: 251287E9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86429-5-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 86429-5-RR (same clone as 86429-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ALDH2 antibody (86429-5-RR, Clone: 251287E9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86429-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86429-5-RR

  Biolayer interferometry (BLl) kinetic assays of 86429-5-RR against Human ALDH2 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

86429-5-PBS cible ALDH2 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ALDH2 Protéine recombinante Ag7452
• Nom complet: aldehyde dehydrogenase 2 family (mitochondrial)
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC002967
• Symbole du gène: ALDH2
• Identification du gène (NCBI): 217
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Acetaldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. It mediates NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. Genetic variation in ALDH2 is responsible for individual differences in responses to drinking alcohol. Thus, the absence of this enzyme is linked to alcohol intolerance and a reduced risk for alcoholism-related liver disease.

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