Anticorps Recombinant de lapin anti-ALDH7A1

• Phare
• Validé par KD/KO

ALDH7A1 Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242363B11

N° de cat : 84871-4-PBS

Synonymes

242363B11, ALDH7A 1, Alpha-AASA dehydrogenase, Alpha-aminoadipic semialdehyde dehydrogenase, Antiquitin-1

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Galerie de données de validation

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  WB analysis using 84871-4-RR (same clone as 84871-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84871-4-RR (ALDH7A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84871-4-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 84871-4-RR (same clone as 84871-4-PBS)

  WB result of ALDH7A1 antibody (84871-4-RR; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH7A1 transfected HepG2 cells. This data was developed using the same antibody clone with 84871-4-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 84871-4-RR (same clone as 84871-4-PBS)

  IP result of anti-ALDH7A1 (IP:84871-4-RR, 4ug; Detection:84871-4-RR 1:3000) with HeLa cells lysate 1200 ug. This data was developed using the same antibody clone with 84871-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84871-4-RR (same clone as 84871-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84871-4-RR (ALDH7A1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84871-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84871-4-RR (same clone as 84871-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84871-4-RR (ALDH7A1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84871-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84871-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84871-4-RR against Human ALDH7A1 were performed. The affinity constant is 0.253 nM.

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Informations sur le produit

84871-4-PBS cible ALDH7A1 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ALDH7A1 Protéine recombinante Ag0383
• Nom complet: aldehyde dehydrogenase 7 family, member A1
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC002515
• Symbole du gène: ALDH7A1
• Identification du gène (NCBI): 501
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ALDH7A1(Alpha-aminoadipic semialdehyde dehydrogenase) is also named as ATQ1 and belongs to the aldehyde dehydrogenase family. It plays a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation.

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