Anticorps Monoclonal anti-ALDOA

ALDOA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1D6C2

N° de cat : 67453-1-PBS

Synonymes

Lung cancer antigen NY-LU-1, Fructose-bisphosphate aldolase A, EC:4.1.2.13, Aldolase A, ALDA

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Galerie de données de validation

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  WB analysis using 67453-1-Ig (same clone as 67453-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67453-1-Ig (ALDOA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67453-1-PBS in a different storage buffer formulation.

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  WB analysis using 67453-1-Ig (same clone as 67453-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67453-1-Ig (ALDOA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67453-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67453-1-Ig (same clone as 67453-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67453-1-Ig (ALDOA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67453-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67453-1-Ig (same clone as 67453-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67453-1-Ig (ALDOA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67453-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67453-1-Ig (same clone as 67453-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ALDOA antibody (67453-1-Ig, Clone: 1D6C2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67453-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67453-1-PBS cible ALDOA dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ALDOA Protéine recombinante Ag17952
• Nom complet: aldolase A, fructose-bisphosphate
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC016800
• Symbole du gène: ALDOA
• Identification du gène (NCBI): 226
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ALDOA(Fructose-bisphosphate aldolase A) is also named as lung cancer antigen NY-LU-1,muscle-type aldolase,ALDA and belongs to the class I fructose-bisphosphate aldolase family.The ALDOA gene encodes a homotetrameric protein(PMID:14615364).It is found in skeletal muscle and red blood cells, and functions in glycolysis, promoting the reversible cleavage of FBP to triose phosphate, G-3-P and DHAP. ALDOA is upregulated in various cancers, including human lung squamous and renal cell carcinomas(PMID:19937139).

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