Anticorps Monoclonal anti-ALDOB

ALDOB Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3D2E4

N° de cat : 68396-1-PBS

Synonymes

ALDB, ALDOB, Liver type aldolase

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Galerie de données de validation

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  WB analysis using 68396-1-Ig (same clone as 68396-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68396-1-Ig (ALDOB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68396-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68396-1-Ig (same clone as 68396-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68396-1-Ig (ALDOB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68396-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68396-1-Ig (same clone as 68396-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68396-1-Ig (ALDOB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68396-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human urine using 68396-1-Ig (same clone as 68396-1-PBS)

  human urine sample were subjected to SDS PAGE followed by western blot with 68396-1-Ig (ALDOB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68396-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 68396-1-Ig (same clone as 68396-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ALDOB antibody (68396-1-Ig, Clone: 3D2E4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68396-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68396-1-PBS cible ALDOB dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, rat, souris

• Réactivité: Humain, Lapin, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ALDOB Protéine recombinante Ag12823
• Nom complet: aldolase B, fructose-bisphosphate
• Masse moléculaire calculée: 316 aa, 35 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC029399
• Symbole du gène: ALDOB
• Identification du gène (NCBI): 229
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Fructose-1,6-bisphosphate aldolase is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate.Vertebrates have 3 aldolase isozymes, aldolase A (ALDOA), B (ALDOB), and C (ALDOC). Deficiency of this enzyme ALDOB causes an accumulation of fructose-1-phosphate after fructose intake, which results in toxic symptoms like vomiting, hypoglycemia, jaundice, elevated liver enzymes and hepatomegaly(PMID:22375183). This antibody may detect ALDOA.

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