Anticorps Polyclonal de lapin anti-Aldolase C

• Phare
• Validé par KD/KO

Aldolase C Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

18

Avis (1)

N° de cat : 14884-1-AP

Synonymes

ALDOC, Aldolase C, Brain type aldolase, Fructose bisphosphate aldolase C

---

Galerie de données de validation

• 

  WB analysis using 14884-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14884-1-AP (Aldolase C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 14884-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 14884-1-AP (Aldolase C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14884-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14884-1-AP (Aldolase C antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14884-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14884-1-AP (Aldolase C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 14884-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14884-1-AP (Aldolase C antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14884-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14884-1-AP (Aldolase C antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 14884-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Aldolase C antibody (14884-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14884-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Aldolase C antibody (14884-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14884-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 14884-1-AP (Aldolase C antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules K-562, tissu cardiaque humain, tissu cérébral de rat, tissu cérébral de souris, tissu de cervelet de souris
• Résultats positifs en IHC: tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

14884-1-AP cible Aldolase C dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Aldolase C Protéine recombinante Ag6659
• Nom complet: aldolase C, fructose-bisphosphate
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC003613
• Symbole du gène: ALDOC
• Identification du gène (NCBI): 230
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fructose-bisphosphate aldolase C (ALDOC) reversibly cleaves FBP and F1-P to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate and is strongly expressed in mammalian brain together with ALDOA and is alsopresent in the heart and spleen of some species(PMID:9363598). It is involved in glycolysis as an important enzyme. Phospholipase D2 and inositol 1,4,5-triphosphate interact with ALDOC in signal transduction. Meanwhile, the protein expression of ALDOC has been reported to be regulated in brain tumor, hepatomas, and lung cancer(PMID:21548097).14884-1-AP can recognize Aldolase A and Aldolase C

Publications

Species	Application	Title
human	WB	Nat Commun; O-GlcNAc modification of leucyl-tRNA synthetase 1 integrates leucine and glucose availability to regulate mTORC1 and the metabolic fate of leucine.; Authors - Kibum Kim
human	WB	Nat Commun; A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism.; Authors - J J David Ho
human	WB	Brain; C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis.; Authors - Scott P Allen
human	IF	Mol Ther; Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes.; Authors - Junyi Hu
mouse	IF	Hum Mol Genet; Lack of CUL4B leads to increased abundance of GFAP-positive cells that is mediated by PTGDS in mouse brain.; Authors - Wei Zhao
human	WB	Stem Cell Reports; The Long Noncoding RNA Lncenc1 Maintains Naive States of Mouse ESCs by Promoting the Glycolysis Pathway.; Authors - Zihao Sun

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Kazu (Verified Customer) (11-22-2022)

This antibody worked on frozen mouse brain tissue sections. A good astrocyte marker.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse brain