Anticorps Polyclonal de lapin anti-ALG1

• Phare
• Validé par KD/KO

ALG1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12872-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 12872-1-AP (same clone as 12872-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12872-1-AP (ALG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 12872-1-AP (same clone as 12872-1-PBS)

  IP result of anti-ALG1 (IP:12872-1-AP, 4ug; Detection:12872-1-AP 1:500) with HeLa cells lysate 3080 ug. This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 12872-1-AP (same clone as 12872-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12872-1-AP (ALG1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 12872-1-AP (same clone as 12872-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12872-1-AP (ALG1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12872-1-AP (same clone as 12872-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12872-1-AP (ALG1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12872-1-PBS cible ALG1 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ALG1 Protéine recombinante Ag3540
• Nom complet: asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae)
• Masse moléculaire calculée: 464 aa, 53 kDa
• Poids moléculaire observé: 47-53 kDa
• Numéro d’acquisition GenBank: BC031095
• Symbole du gène: ALG1
• Identification du gène (NCBI): 56052
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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