Anticorps Monoclonal anti-Alix

• Phare
• Validé par KD/KO

Alix Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(18)

CloneNo.

1H9D9

N° de cat : 67715-1-Ig

Synonymes

PDCD6IP, 1H9D9, AIP1, ALG-2-interacting protein 1, ALG-2-interacting protein X

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Galerie de données de validation

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  WB analysis using 67715-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67715-1-Ig (Alix antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67715-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67715-1-Ig (Alix antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67715-1-Ig

  WB result of Alix antibody (67715-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alix transfected HEK-293 cells.

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  IHC staining of human stomach cancer using 67715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67715-1-Ig (Alix antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 67715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67715-1-Ig (Alix antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67715-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Alix antibody (67715-1-Ig, Clone: 1H9D9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HSC-T6, cellules 4T1, cellules A431, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules PC-12
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:20000
• Immunohistochimie (IHC): IHC : 1:4000-1:16000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 16 publications below
• IF: See 1 publications below

Informations sur le produit

67715-1-Ig cible Alix dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, singe, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Alix Protéine recombinante Ag30437
• Nom complet: programmed cell death 6 interacting protein
• Masse moléculaire calculée: 868 aa, 96 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC020066
• Symbole du gène: Alix
• Identification du gène (NCBI): 10015
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium.

Publications

Species	Application	Title
human	WB	Stem Cell Res Ther; Tremella polysaccharide microneedles loaded with magnetic dental pulp stem cell intracellular vesicles used for androgenic alopecia; Authors - Yonghao Liu
human,mouse	WB,IF	Environ Pollut; TDCPP Disrupts ALG-2/ALIX-Mediated ESCRT-III Recruitment: Implications for Lysosomal Membrane Repair and Neurotoxicity; Authors - Tingting Li
human	WB	Cell Mol Biol Lett; Downregulation of exosomal miR-7-5p promotes breast cancer migration and invasion by targeting RYK and participating in the atypical WNT signalling pathway; Authors - Zhaoyi Liang
mouse,human	WB	J Thromb Haemost; Cancer-associated fibroblasts promote venous thrombosis through podoplanin/CLEC-2 interaction in podoplanin-negative lung cancer mouse model; Authors - Toshiaki Shirai
human	WB	Psychiatry Clin Neurosci; Human in vivo evidence of reduced astrocyte activation and neuroinflammation in patients with treatment-resistant depression following electroconvulsive therapy; Authors - Shu-Xian Xu
human	WB	J Microsc; A method for extraction of exosomes from breast tumour cells and characterisation by transmission electron microscopy; Authors - Zhiang Liu

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