Anticorps Polyclonal de lapin anti-ALKBH2

ALKBH2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 17809-1-AP

Synonymes

ABH2, ALKBH2, Oxy DC1

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Galerie de données de validation

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  WB analysis using 17809-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17809-1-AP (ALKBH2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Caco-2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

17809-1-AP cible ALKBH2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ALKBH2 Protéine recombinante Ag12108
• Nom complet: alkB, alkylation repair homolog 2 (E. coli)
• Masse moléculaire calculée: 261 aa, 29 kDa
• Poids moléculaire observé: 30-35 kDa
• Numéro d’acquisition GenBank: BC070489
• Symbole du gène: ALKBH2
• Identification du gène (NCBI): 121642
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Escherichia coli AlkB is a DNA repair enzyme that represents the founding member of the AlkB family of non-heme iron-dependent dioxygenases. The human genome encodes nine AlkB homologs termed AlkB homolog 1 through 8 (ALKBH1 through 8) plus a ninth member termed fat mass and obesity associated (FTO). Bacterial AlkB as well as human ALKBH2 and ALKBH3 catalyze the direct reversal of DNA methylation damage through a distinct oxidative demethylation reaction. Importantly, ALKBH2 has been shown to play a role in DNA alkylation damage repair in human, mouse and plant cells. Full-length ALKBH2 has a molecular weight of about 30kDa and ALKBH2 variant encoded by an alternatively spliced transcript with a frameshift deletion of the C-terminal dioxygenase domain has a molecular weight of about 25kDa (PMID:22079122).

Publications

Species	Application	Title
human	WB	Cancer Gene Ther; Pan-cancer oncogenic properties and therapeutic potential of SF3B4; Authors - Yanmei Shi

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