Anticorps Polyclonal de lapin anti-ALKBH3

• Phare
• Validé par KD/KO

ALKBH3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12292-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 12292-1-AP (same clone as 12292-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12292-1-AP (ALKBH3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 12292-1-AP (same clone as 12292-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12292-1-AP (ALKBH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 12292-1-AP (same clone as 12292-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12292-1-AP (ALKBH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12292-1-AP (same clone as 12292-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12292-1-AP (ALKBH3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 12292-1-AP (same clone as 12292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12292-1-AP (ALKBH3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 12292-1-AP (same clone as 12292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 12292-1-AP (same clone as 12292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 12292-1-AP (same clone as 12292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

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  IHC staining of rat stomach using 12292-1-AP (same clone as 12292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 12292-1-AP (same clone as 12292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 12292-1-AP (same clone as 12292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 12292-1-AP (ALKBH3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 12292-1-AP (same clone as 12292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 12292-1-AP (ALKBH3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12292-1-PBS cible ALKBH3 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ALKBH3 Protéine recombinante Ag2982
• Nom complet: alkB, alkylation repair homolog 3 (E. coli)
• Masse moléculaire calculée: 286 aa, 33 kDa
• Poids moléculaire observé: 22-25 kDa, 33kDa
• Numéro d’acquisition GenBank: BC015155
• Symbole du gène: ALKBH3
• Identification du gène (NCBI): 221120
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ALKBH3, also named as ABH3 and DEPC-1, is a dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). It has a strong preference for single-stranded. ALKBH3 is requires molecular oxygen, alpha-ketoglutarate and iron. This gene has some isoforms with MW 19-25 kDa and 33 kDa.

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