Anticorps Recombinant de lapin anti-ALKBH3

ALKBH3 Recombinant Antibody for WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

241650F10

N° de cat : 84314-2-PBS

Synonymes

241650F10, ABH3, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, DEPC 1, DEPC1

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Galerie de données de validation

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  WB analysis using 84314-2-RR (same clone as 84314-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84314-2-RR (ALKBH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84314-2-PBS in a different storage buffer formulation.

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  WB analysis using 84314-2-RR (same clone as 84314-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84314-2-RR (ALKBH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84314-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01211-2

  Sandwich ELISA standard curve of MP01211-2, Human ALKBH3 Recombinant Matched Antibody Pair - PBS only. 84314-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2982. 84314-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  Sample test of MP01211-2

  The mean ALKBH3 concentration was determined to be 6.80 ng/mL in Jurkat cell extract based on a 1.50 mg/mL extract load.

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  FC experiment of HeLa using 84314-2-RR (same clone as 84314-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ALKBH3 Recombinant antibody (84314-2-RR, Clone:241650F10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84314-2-PBS in a different storage buffer formulation.

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  FC experiment of PC-3 using 84314-2-RR (same clone as 84314-2-PBS)

  1x10^6 PC-3 cells were intracellularly stained with 0.25 ug ALKBH3 Recombinant antibody (84314-2-RR, Clone:241650F10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84314-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84314-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 84314-2-PBS against Human ALKBH3 was performed. The affinity constant is 0.59 nM.

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  Affinity and Kinetic Characterization of 84314-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84314-2-RR against Human ALKBH3 were performed. The affinity constant is 1.99 nM.

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Informations sur le produit

84314-2-PBS cible ALKBH3 dans les applications de WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ALKBH3 Protéine recombinante Ag2982
• Nom complet: alkB, alkylation repair homolog 3 (E. coli)
• Masse moléculaire calculée: 286 aa, 33 kDa
• Poids moléculaire observé: 30-33 kDa
• Numéro d’acquisition GenBank: BC015155
• Symbole du gène: ALKBH3
• Identification du gène (NCBI): 221120
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ALKBH3, also named as ABH3 and DEPC-1, is a dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). It has a strong preference for single-stranded. ALKBH3 is requires molecular oxygen, alpha-ketoglutarate and iron. This gene has some isoforms with MW 19-25 kDa and 33 kDa.

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