Anticorps Polyclonal de lapin anti-ALKBH5

• Phare
• Validé par KD/KO

ALKBH5 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(195)

Avis (5)

N° de cat : 16837-1-AP

Synonymes

ALKBH 5, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5, EC:1.14.11.53, RNA demethylase ALKBH5

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Galerie de données de validation

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  WB analysis using 16837-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16837-1-AP (ALKBH5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16837-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16837-1-AP (ALKBH5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16837-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16837-1-AP (ALKBH5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16837-1-AP

  IP result of anti-ALKBH5 (IP:16837-1-AP, 4ug; Detection:16837-1-AP 1:8000) with HeLa cells lysate 1280 ug.

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  IHC staining of human testis using 16837-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16837-1-AP (ALKBH5 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 16837-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16837-1-AP (ALKBH5 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse testis using 16837-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16837-1-AP (ALKBH5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 16837-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16837-1-AP (ALKBH5 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 16837-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16837-1-AP (ALKBH5 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu testiculaire humain, tissu de cancer du poumon humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 19 publications below
• WB: See 173 publications below
• IHC: See 26 publications below
• IF: See 16 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• RIP: See 17 publications below

Informations sur le produit

16837-1-AP cible ALKBH5 dans les applications de WB, IHC, IF, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ALKBH5 Protéine recombinante Ag10516
• Nom complet: alkB, alkylation repair homolog 5 (E. coli)
• Masse moléculaire calculée: 458 aa, 51 kDa
• Poids moléculaire observé: 40-50 kDa
• Numéro d’acquisition GenBank: BC062339
• Symbole du gène: ALKBH5
• Identification du gène (NCBI): 54890
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.

Publications

Species	Application	Title
human	WB	Nat Genet; N6-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2.; Authors - Yuan Li
human	IF	Cell Res; The m6A methylome of SARS-CoV-2 in host cells.; Authors - Jun'e Liu ; KD Validated
mouse	WB,RIP	Nat Cell Biol; The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N6-methyladenosine methylation of Parp10 mRNA.; Authors - Xiang-Qian Gao
human	WB	Nat Commun; Circular RNA encoded MET variant promotes glioblastoma tumorigenesis; Authors - Jian Zhong
mouse,human	WB	Nat Commun; Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant glioma; Authors - Weiwei Dai
mouse	WB	Nat Commun; ALKBH5-mediated m6A modification of IL-11 drives macrophage-to-myofibroblast transition and pathological cardiac fibrosis in mice; Authors - Tao Zhuang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

PK (Verified Customer) (07-08-2022)

GOOD

• Applications: Western Blot
• Cell Tissue Type: H9C2

ALKBH5 Antibody Western Blot validation ( dilution) in H9C2 (Cat no:16837-1-AP)

FH

SU (Verified Customer) (03-22-2022)

• Applications: Western Blot
• Cell Tissue Type: H9C2

ALKBH5 Antibody Western Blot validation ( dilution) in H9C2 (Cat no:16837-1-AP)

FH

ANIRBAN (Verified Customer) (04-07-2021)

Tried to validate the antibody (AlkBH5) in two different cell lines (786-o, HK2) using three siRNA constructs from IDT. Using the Proteintech antibody, I didn't see any evedence of KD in the lysates (see the figure attached). When I used the Cell Signaling antibody for AlkBH5 (#80283) I observed a clear evedence of KD in the same lysates that I mentioned before. scr=scrambled; si-1,2,3=siRNAs targeting AlkBH5.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2K
• Cell Tissue Type: RCC cell lines

ALKBH5 Antibody Western Blot validation (1:2K dilution) in RCC cell lines (Cat no:16837-1-AP)

FH

Bill (Verified Customer) (12-23-2019)

This antibody has some unspecific band, but is ok to detect ALKBH5, siRNA validated.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: colerectal cancer cells

FH

Daniel (Verified Customer) (12-04-2019)

Staining of kidney proximal tubules

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse kidney (TRACK model)

ALKBH5 Antibody Immunohistochemistry, validation (1:200 dilution) in Mouse kidney (TRACK model) (Cat no:16837-1-AP)

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