Anticorps Monoclonal anti-ALKBH5

• Phare
• Validé par KD/KO

ALKBH5 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D2B2

N° de cat : 67811-1-PBS

Synonymes

ABH5, ALKBH5, FLJ20308, OFOXD, OFOXD1

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Galerie de données de validation

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  WB analysis of HSC-T6 using 67811-1-Ig (same clone as 67811-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67811-1-Ig (same clone as 67811-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67811-1-Ig (same clone as 67811-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67811-1-Ig (same clone as 67811-1-PBS)

  WB result of ALKBH5 antibody (67811-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALKBH5 transfected HeLa cells. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67811-1-Ig (same clone as 67811-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67811-1-Ig (same clone as 67811-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 67811-1-Ig (same clone as 67811-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 67811-1-Ig (same clone as 67811-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67811-1-Ig (ALKBH5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 67811-1-Ig (same clone as 67811-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67811-1-Ig (ALKBH5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67811-1-PBS cible ALKBH5 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ALKBH5 Protéine recombinante Ag10490
• Nom complet: alkB, alkylation repair homolog 5 (E. coli)
• Masse moléculaire calculée: 458 aa, 51 kDa
• Poids moléculaire observé: 40-44 kDa
• Numéro d’acquisition GenBank: BC062339
• Symbole du gène: ALKBH5
• Identification du gène (NCBI): 54890
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.

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