Anticorps Polyclonal de lapin anti-ALKBH8

ALKBH8 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25566-1-PBS

Synonymes

ABH8, EC:2.1.1.229, Probable alpha-ketoglutarate-dependent dioxygenase ABH8, S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8, tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8

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Galerie de données de validation

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  IHC staining of rat kidney using 25566-1-AP (same clone as 25566-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 25566-1-AP (ALKBH8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25566-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 25566-1-AP (same clone as 25566-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using ALKBH8 antibody (25566-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 25566-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 25566-1-AP (same clone as 25566-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ALKBH8 antibody (25566-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 25566-1-PBS in a different storage buffer formulation.

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Informations sur le produit

25566-1-PBS cible ALKBH8 dans les applications de IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ALKBH8 Protéine recombinante Ag22239
• Nom complet: alkB, alkylation repair homolog 8 (E. coli)
• Masse moléculaire calculée: 664 aa, 75 kDa
• Numéro d’acquisition GenBank: BC015183
• Symbole du gène: ALKBH8
• Identification du gène (NCBI): 91801
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ALKBH8 (Alpha-ketoglutarate-dependent dioxygenase) is unique among the ALKBH family, since it harbors a 2-oxoglutarate and Fe(II)-dependent oxygenase domain as well as an RNA recognition motif and methyl transferase domain (PMID: 27329810). ALKBH8 is a "writer" enzyme that modifies the wobble uridine of tRNA and regulates protein translation, including that of selenoproteins (PMID: 38550277). ALKBH8 is highly expressed in high-grade, superficially and deeply invasive bladder cancer. ALKBH8 knockdown induces apoptosis in bladder cancer cells (PMID: 38550277).

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