Anticorps Polyclonal de lapin anti-ALOX15B

ALOX15B Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(10)

Avis (2)

N° de cat : 13073-1-AP

Synonymes

15 lipoxygenase 2, 15-LOX-2, 15-lipoxygenase 2, 15 LOX B, 15 LOX 2

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Galerie de données de validation

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  WB analysis of A431 using 13073-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13073-1-AP (15 Lipoxygenase 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13073-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13073-1-AP (15 Lipoxygenase 2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HeLa, cellules HL-60, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IHC: See 1 publications below

Informations sur le produit

13073-1-AP cible ALOX15B dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ALOX15B Protéine recombinante Ag3741
• Nom complet: arachidonate 15-lipoxygenase, type B
• Masse moléculaire calculée: 676 aa, 76 kDa
• Poids moléculaire observé: 67-76 kDa
• Numéro d’acquisition GenBank: BC035217
• Symbole du gène: ALOX15B
• Identification du gène (NCBI): 247
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

In humans, there are 6 functional lipoxygenases genes. Two of them encode for arachidonic acid 12-lipoxygenating (ALOX12, ALOX12B) and two for 15-lipoxygenating enzymes (ALOX15, ALOX15B). ALOX15B has been described as the most abundant 15-lipoxygenating enzyme species in human carotid atherosclerotic lesions and to be associated with cerebrovascular symptoms. (PMID:24373925). ALOX15B has 4 isoforms produced by alternative splicing with the MW of 76, 67, 69 and 73 kDa. 13073-1-AP can also recognize the mouse Alox8 protein.

Publications

Species	Application	Title
	WB	Nutrition; Intermittent fasting ameliorates neuronal ferroptosis and cognitive impairment in mice after traumatic brain injury; Authors - Qiuyun Yang
rat	IHC	Exp Cell Res; Lipoxin A4 and its analog attenuate high fat diet-induced atherosclerosis via Keap1/Nrf2 pathway.; Authors - Fen Xu
	WB	J Chem Neuroanat; Proanthocyanidin promotes functional recovery of spinal cord injury via inhibiting ferroptosis.; Authors - Huangao Zhou
pig	WB	Theriogenology; The role of ALOX15B in heat stress-induced apoptosis of porcine sertoli cells.; Authors - Hongyan Xue
human	WB	Redox Biol; Excessive SOX8 reprograms energy and iron metabolism to prime hepatocellular carcinoma for ferroptosis; Authors - Xue Yang
human	WB	Lipids Health Dis; Lipid peroxidation-induced ferroptosis as a therapeutic target for mitigating neuronal injury and inflammation in sepsis-associated encephalopathy: insights into the hippocampal PEBP-1/15-LOX/GPX4 pathway; Authors - Haosen Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nazek (Verified Customer) (08-07-2025)

some non-specific bands seen in the western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:500

ALOX15B Antibody Western Blot validation (1:500 dilution) in (Cat no:13073-1-AP)

FH

Ankush (Verified Customer) (04-23-2024)

The antibody showed slightly different position. please refer to the marker lane

• Applications: Immunoprecipitation
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: monocytes

ALOX15B Antibody Immunoprecipitation validation (1:2500 dilution) in monocytes (Cat no:13073-1-AP)

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