Anticorps Recombinant de lapin anti-ALOX15B

ALOX15B Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Cytometric bead array, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

250442C5

N° de cat : 86146-1-PBS

Synonymes

15 lipoxygenase 2, 15 LO 2, 15 LOX 2, 15 LOX B, 15-lipoxygenase 2

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Galerie de données de validation

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  WB analysis using 86146-1-RR (same clone as 86146-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 86146-1-RR (ALOX15B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86146-1-PBS in a different storage buffer formulation.

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  Sample test of MP02510-1

  The mean ALOX15B concentration was determined to be 28.7 ng/mL in A431 cell extract based on a 1.6 mg/mL extract load, 52.4 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load.

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  Cytometric bead array standard curve of MP02510-1

  Cytometric bead array standard curve of MP02510-1, ALOX15B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86146-1-PBS. Detection antibody: 86146-2-PBS. Standard: Ag3741. Range: 0.938-60 ng/mL.

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  Affinity and Kinetic Characterization of 86146-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86146-1-RR against Human ALOX15B were performed. The affinity constant is below 1 pM.

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Informations sur le produit

86146-1-PBS cible ALOX15B dans les applications de WB, Cytometric bead array, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ALOX15B Protéine recombinante Ag3741
• Nom complet: arachidonate 15-lipoxygenase, type B
• Masse moléculaire calculée: 676 aa, 76 kDa
• Poids moléculaire observé: 67-75 kDa
• Numéro d’acquisition GenBank: BC035217
• Symbole du gène: ALOX15B
• Identification du gène (NCBI): 247
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

In humans, there are 6 functional lipoxygenases genes. Two of them encode for arachidonic acid 12-lipoxygenating (ALOX12, ALOX12B) and two for 15-lipoxygenating enzymes (ALOX15, ALOX15B). ALOX15B has been described as the most abundant 15-lipoxygenating enzyme species in human carotid atherosclerotic lesions and to be associated with cerebrovascular symptoms. (PMID:24373925).

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