Anticorps Recombinant de lapin anti-5 Lipoxygenase

5 Lipoxygenase Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

240740A10

N° de cat : 83794-4-PBS

Synonymes

ALOX5, 5-LO, 5LO, 5-lipoxygenase, 5 LOX

---

Galerie de données de validation

• 

  WB analysis using 83794-4-RR (same clone as 83794-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83794-4-RR (ALOX5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83794-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovarian cancer using 83794-4-RR (same clone as 83794-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83794-4-RR (5 Lipoxygenase antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83794-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83794-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83794-4-RR against Human 5 Lipoxygenase were performed. The affinity constant is 0.392 nM.

---

Informations sur le produit

83794-4-PBS cible 5 Lipoxygenase dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: 5 Lipoxygenase Protéine recombinante Ag19503
• Nom complet: arachidonate 5-lipoxygenase
• Masse moléculaire calculée: 674 aa, 78 kDa
• Poids moléculaire observé: 70-78 kDa
• Numéro d’acquisition GenBank: BC130332
• Symbole du gène: 5 Lipoxygenase
• Identification du gène (NCBI): 240
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).

{{ptg:RelatedPrimaryAntibodies}}