Anticorps Monoclonal anti-ALPK1

ALPK1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B8B5

N° de cat : 67689-1-PBS

Synonymes

2B8B5, alpha kinase 1, Alpha protein kinase 1, Alpha-protein kinase 1, ALPK

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Galerie de données de validation

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  WB analysis using 67689-1-Ig (same clone as 67689-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67689-1-Ig (ALPK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67689-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 67689-1-Ig (same clone as 67689-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67689-1-Ig (ALPK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67689-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 67689-1-Ig (same clone as 67689-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67689-1-Ig (ALPK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67689-1-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 67689-1-Ig (same clone as 67689-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using ALPK1 antibody (67689-1-Ig, Clone: 2B8B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67689-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67689-1-PBS cible ALPK1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ALPK1 Protéine recombinante Ag5320
• Nom complet: alpha-kinase 1
• Masse moléculaire calculée: 139 kDa
• Poids moléculaire observé: 139 kDa
• Numéro d’acquisition GenBank: BC060780
• Symbole du gène: ALPK1
• Identification du gène (NCBI): 80216
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Alpha-protein kinase 1, also known as alpha-kinase 1 (ALPK1), is associated with chronic kidney disease (CKD), myocardial infarction, gout and type 2 diabetes mellitus (DM). ALPK1 mutant has been reported to cause severe defects in motor co‐ordination in mice. ALPK1 enhances the expression of proinflammatory cytokines including IL-1β, IL-8 and TGF-β1 in cultured human THP1 and human embryonic kidney 293 (HEK293) cells (PMID: 31557402, PMID: 26275947). ALPK1 short isoform (108 kDa) was highly expressed in brain, spinal cord, heart, lung, spleen, thymus, small intestine, skin and testis, while detectable inskeletal muscle and kidney. ALPK1 large isoform (130 kDa) appeared in heart, lung, thymus and skin (PMID: 21208416).

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