Anticorps Monoclonal anti-ALPL

ALPL Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, porc

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E3C1

N° de cat : 68719-1-PBS

Synonymes

Alkaline phosphatase, 2E3C1, AKP2, Alkaline phosphatase liver/bone/kidney isozyme, Alkaline phosphatase tissue nonspecific isozyme

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Galerie de données de validation

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  WB analysis using 68719-1-Ig (same clone as 68719-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68719-1-Ig (ALPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68719-1-PBS in a different storage buffer formulation.

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  IHC staining of rat adrenal gland using 68719-1-Ig (same clone as 68719-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 68719-1-Ig (ALPL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68719-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68719-1-PBS cible ALPL dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc

• Réactivité: Humain, Lapin, porc
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ALPL Protéine recombinante Ag33678
• Nom complet: alkaline phosphatase, liver/bone/kidney
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC021289
• Symbole du gène: ALPL
• Identification du gène (NCBI): 249
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. It can exsit as a homodimer with the molecular weight of 140 kDa(PMID:18724009). ALPL was synthesized as a 66-kDa endo-b-N-acetylglucos-aminidase H (Endo H)-sensitive form, and processed to an 80-kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol (GPI)(PMID:10839996).

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