Anticorps Recombinant de lapin anti-ALR

ALR Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242557G3

N° de cat : 84972-4-PBS

Synonymes

GFER, 242557G3, EC:1.8.3.2, ERV1, FAD-linked sulfhydryl oxidase ALR

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Galerie de données de validation

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  WB analysis using 84972-4-RR (same clone as 84972-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84972-4-RR (GFER antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84972-4-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84972-4-RR (same clone as 84972-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84972-4-RR (ALR antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84972-4-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 84972-4-RR (same clone as 84972-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 84972-4-RR (ALR antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84972-4-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 84972-4-RR (same clone as 84972-4-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ALR Recombinant antibody (84972-4-RR, Clone:242557G3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84972-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84972-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84972-4-RR against Human ALR were performed. The affinity constant is 0.23 nM.

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Informations sur le produit

84972-4-PBS cible ALR dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ALR Protéine recombinante Ag1840
• Nom complet: growth factor, augmenter of liver regeneration
• Masse moléculaire calculée: 15 kDa, 23 kDa
• Poids moléculaire observé: 23-25 kDa
• Numéro d’acquisition GenBank: BC028348
• Symbole du gène: GFER
• Identification du gène (NCBI): 2671
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GFER (FAD-linked sulfhydryl oxidase) is also named as ALR, HERV1, HPO. It plays an important role in the disulfide relay system (DRS) in human mitochondria. The GFER gene codes for 2 distinct isoforms that are probably synthesized from the same mRNA with the use of different initiation codons. The long isoform (205 amino acids, 23/21 kD) is located mainly in the mitochondrial intermembrane space and exists under nonreducing and nondenaturing conditions as a homodimer and a heterodimer. The shorter isoform (125 amino acids, 15 kD), which lacks 80 amino acids at its N terminus compared to the longer isoform, is present predominantly in the nucleus (PMID: 19409522, 24880092, 21152698).

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