Anticorps Recombinant de lapin anti-AMH

AMH Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

241148C8

N° de cat : 84153-5-RR

Synonymes

Muellerian inhibiting factor, Anti-Muellerian hormone (AMH), Anti-Muellerian hormone, anti Mullerian hormone, Anti muellerian hormone

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Galerie de données de validation

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  WB analysis using 84153-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84153-5-RR (AMH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 84153-5-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84153-5-RR (AMH antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 84153-5-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84153-5-RR (AMH antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84153-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84153-5-RR against Human AMH were performed. The affinity constant is 2.16 nM.

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Applications testées

• Résultats positifs en WB: cellules LNCaP, tissu ovarien de souris
• Résultats positifs en IHC: human ovary cancer tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84153-5-RR cible AMH dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: AMH Protéine recombinante Ag5670
• Nom complet: anti-Mullerian hormone
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 60-65 kDa
• Numéro d’acquisition GenBank: BC049194
• Symbole du gène: AMH
• Identification du gène (NCBI): 268
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Anti-Müllerian hormone (AMH), also called Müllerian-inhibiting substance (MIS), is a hormone that is best known for its production by fetal testes in mammals and as the inhibitor of Müllerian duct development in males. AMH is also expressed in granulosa cells of preantral and small antral follicles in the ovary. More recently, AMH has been studied for its role in ovarian folliculogenesis and as a potential marker of ovarian reserve. The deduced protein sequence of the AMH monomer contains a 25 amino acid secretion specific signal peptide and a monomeric protein of 535 amino acids that, when glycosylated at two glycosylation sites, has a molecular weight of 65-70 kDa.

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