Anticorps Polyclonal de lapin anti-AMN1

AMN1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14694-1-PBS

Synonymes

AMN 1

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Galerie de données de validation

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  WB analysis of human colon using 14694-1-AP (same clone as 14694-1-PBS)

  human colon tissue were subjected to SDS PAGE followed by western blot with 14694-1-AP (AMN1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14694-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 14694-1-AP (same clone as 14694-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14694-1-AP (AMN1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14694-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14694-1-PBS cible AMN1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AMN1 Protéine recombinante Ag6257
• Nom complet: antagonist of mitotic exit network 1 homolog (S. cerevisiae)
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC067906
• Symbole du gène: AMN1
• Identification du gène (NCBI): 196394
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AMN1, also known as the antagonist of mitotic exit network 1 homolog, is a protein-coding gene that plays a significant role in regulating cell division and mitotic exit in Saccharomyces cerevisiae (baker's yeast). AMN1 is an atypical F-box protein involved in the regulation of the MEN pathway. It acts as an antagonist by disrupting the formation of the Tem1/Cdc15 complex, which is crucial for mitotic exit. Specifically, AMN1 competes with Cdc15 for binding to Tem1, thereby turning off the MEN pathway. AMN1 is essential for cell separation after cytokinesis. It inhibits the transcription factor Ace2, leading to the downregulation of Ace2 target genes. This inhibition prevents septum cleavage and results in post-mitotic cell separation inhibition, causing cell clumping.

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