Anticorps Monoclonal anti-AMOTL2

AMOTL2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2C4A4

N° de cat : 68401-1-PBS

Synonymes

AMOTL2, angiomotin like 2, Angiomotin like protein 2, KIAA0989, LCCP, Leman coiled coil protein

---

Galerie de données de validation

• 

  WB analysis using 68401-1-Ig (same clone as 68401-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68401-1-Ig (AMOTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68401-1-PBS in a different storage buffer formulation.

• 

  WB analysis of DU 145 using 68401-1-Ig (same clone as 68401-1-PBS)

  DU 145 cells were subjected to SDS PAGE followed by western blot with 68401-1-Ig (AMOTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68401-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Saos-2 using 68401-1-Ig (same clone as 68401-1-PBS)

  Saos-2 cells were subjected to SDS PAGE followed by western blot with 68401-1-Ig (AMOTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68401-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NCI-H1299 using 68401-1-Ig (same clone as 68401-1-PBS)

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 68401-1-Ig (AMOTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68401-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 68401-1-Ig (same clone as 68401-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using AMOTL2 antibody (68401-1-Ig, Clone: 2C4A4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68401-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

68401-1-PBS cible AMOTL2 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AMOTL2 Protéine recombinante Ag19971
• Nom complet: angiomotin like 2
• Masse moléculaire calculée: 779 aa, 86 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC011454
• Symbole du gène: AMOTL2
• Identification du gène (NCBI): 51421
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Angiomotin‑like 2 (AMOTL2) is a member of the motin family of angiostatin‑binding proteins. The motin family, also known as AMOTs, consists of three members: AMOT, AMOT-like 1 (AMOTL1) and AMOTL2. Members of the motin family are a type of adaptor proteins mainly distributed in the cytomembrane, cytoplasm or nucleus, and have a higher expression in the endothelial cells of capillaries and in larger vessels of the placenta. Human AmotL2 encodes two isoforms of a molecular mass of 100 kDa and 60 kDa.(PMID: 34036399,PMID: 25080976)

{{ptg:RelatedPrimaryAntibodies}}