Anticorps Polyclonal de lapin anti-Amphiphysin

• Phare
• Validé par KD/KO

Amphiphysin Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 13379-1-AP

Synonymes

AMPH, AMPH1, Amphiphysin

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Galerie de données de validation

• 

  WB analysis of mouse brain using 13379-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13379-1-AP (Amphiphysin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 13379-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13379-1-AP (Amphiphysin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 13379-1-AP

  IP Result of anti-Amphiphysin (IP:13379-1-AP, 4ug; Detection:13379-1-AP 1:1500) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of mouse brain using 13379-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13379-1-AP (Amphiphysin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13379-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13379-1-AP (Amphiphysin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 13379-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13379-1-AP (Amphiphysin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 13379-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13379-1-AP (Amphiphysin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 13379-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13379-1-AP (Amphiphysin antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 13379-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13379-1-AP (Amphiphysin antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu pulmonaire de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cancer du pancréas humain, tissu de cervelet de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 7 publications below
• IF: See 5 publications below

Informations sur le produit

13379-1-AP cible Amphiphysin dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Amphiphysin Protéine recombinante Ag4204
• Nom complet: amphiphysin
• Masse moléculaire calculée: 695 aa, 76 kDa
• Poids moléculaire observé: 115-125 kDa
• Numéro d’acquisition GenBank: BC034376
• Symbole du gène: AMPH
• Identification du gène (NCBI): 273
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Amphiphysin (AMPH), a synaptic vesicle-associated protein that is highly concentrated in nerve terminal, is an autoantigen of Stiff-Man syndrome with breast cancer. Amphiphysin contains a N-terminal BAR (Bin1/Amphiphysin/Rv167) domain followed by an endocytosis domain, and a C-terminal SH3 domain. Amphiphysin has been proposed to function as a linker between the clathrin coat and dynamin in the endocytosis of synaptic vesicles. It may also participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. (PMID: 8552632; 8245793; 10559861)

Publications

Species	Application	Title
mouse	WB,IHC	Nat Cell Biol; Stromal-epithelial crosstalk regulates kidney progenitor cell differentiation.; Authors - Das Amrita A
mouse	IHC	Development; Canonical Wnt9b signaling balances progenitor cell expansion and differentiation during kidney development.; Authors - Karner Courtney M CM
mouse	WB	Aging (Albany NY); Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis.; Authors - Dongdong Wang
mouse	WB	Aging (Albany NY); A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.; Authors - Xiao Wu
mouse	WB	FASEB J; PTENα regulates endocytosis and modulates olfactory function.; Authors - Yuyao Yuan
human	WB,IHC	J Cell Mol Med; AMPH1 functions as a tumour suppressor in ovarian cancer via the inactivation of PI3K/AKT pathway.; Authors - Yajun Chen ; KD Validated

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