Anticorps Monoclonal anti-AMPK Beta 2

AMPK Beta 2 Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

2D5C1

N° de cat : 66579-1-Ig

Synonymes

AMPK subunit beta 2, PRKAB2

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Galerie de données de validation

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  WB analysis using 66579-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66579-1-Ig (AMPK beta 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 66579-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66579-1-Ig (AMPK beta 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66579-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66579-1-Ig (AMPK beta 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 66579-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66579-1-Ig (AMPK beta 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 66579-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AMPK Beta 2 antibody (66579-1-Ig, Clone: 2D5C1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules A431, cellules A549, cellules HeLa, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66579-1-Ig cible AMPK Beta 2 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AMPK Beta 2 Protéine recombinante Ag5806
• Nom complet: protein kinase, AMP-activated, beta 2 non-catalytic subunit
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC053610
• Symbole du gène: PRKAB2
• Identification du gène (NCBI): 5565
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AMPK beta 2 is a regulatory subunit of the AMP-activated protein kinase (AMPK), which is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK beta 2 may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles.