Anticorps Monoclonal anti-AMT

AMT Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3E7D4

N° de cat : 67532-1-PBS

Synonymes

GCST, 3E7D4, aminomethyltransferase, EC:2.1.2.10, GCVT

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Galerie de données de validation

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  WB analysis of pig liver using 67532-1-Ig (same clone as 67532-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67532-1-Ig (AMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 67532-1-Ig (same clone as 67532-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67532-1-Ig (AMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 67532-1-Ig (same clone as 67532-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67532-1-Ig (AMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67532-1-Ig (same clone as 67532-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67532-1-Ig (AMT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67532-1-Ig (same clone as 67532-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67532-1-Ig (AMT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67532-1-Ig (same clone as 67532-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AMT antibody (67532-1-Ig, Clone: 3E7D4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50403-2

  Cytometric bead array standard curve of MP50403-2, AMT Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67532-3-PBS. Detection antibody: 67532-1-PBS. Standard:Ag26644. Range: 3.125-100 ng/mL.

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  Affinity and Kinetic Characterization of 67532-1-Ig

  Biolayer interferometry (BLl) kinetic assay of 67532-1-Ig against Human AMT was performed. The affinity constant is 8.85 nM.

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Informations sur le produit

67532-1-PBS cible AMT dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AMT Protéine recombinante Ag26644
• Nom complet: aminomethyltransferase
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 37-44 kDa
• Numéro d’acquisition GenBank: BC007546
• Symbole du gène: AMT
• Identification du gène (NCBI): 275
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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