Anticorps Polyclonal de lapin anti-Amylase Alpha

Amylase Alpha Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(18)

Avis (4)

N° de cat : 12540-1-AP

Synonymes

AMY2B, Amylase, alpha, 1,4-alpha-D-glucan glucanohydrolase 2B, Alpha amylase, Alpha amylase 2B

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Galerie de données de validation

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  WB analysis of mouse pancreas using 12540-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12540-1-AP (Amylase, alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 12540-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12540-1-AP (Amylase, alpha antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12540-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12540-1-AP (Amylase, alpha antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat pancreas using 12540-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat pancreas tissue using Amylase Alpha antibody (12540-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu pancréatique de souris,
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu pancréatique de rat,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 6 publications below
• IF: See 9 publications below

Informations sur le produit

12540-1-AP cible Amylase Alpha dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Amylase Alpha Protéine recombinante Ag3260
• Nom complet: amylase, alpha 2B (pancreatic)
• Masse moléculaire calculée: 511 aa, 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC011179
• Symbole du gène: AMY2B
• Identification du gène (NCBI): 280
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AMY2B(alpha-amylase 2B) belongs to the glycosyl hydrolase 13 family. This protein is involved in the endohydrolysis of 1,4-alpha-glucosidic linkages in oligosaccharides and polysaccharides. The full length 58 kDa protein has a signal peptide with 15 amino acids. Human amylases (Amy1, Amy2A, and Amy2B) commonly have two potential N-glycosylation sites (N427 and N476) in their C-terminal region (S Takashima, J Amano, 2012). 12540-1-AP can recognize both AMY2A and AMY2B.

Publications

Species	Application	Title
mouse	IF	ACS Nano; Inflammation and Acinar Cell Dual-Targeting Nanomedicines for Synergistic Treatment of Acute Pancreatitis via Ca2+ Homeostasis Regulation and Pancreas Autodigestion Inhibition; Authors - Yanan Wang
mouse	IF	Cancer Lett; SULF2 enhances GDF15-SMAD axis to facilitate the initiation and progression of pancreatic cancer.; Authors - Ruizhe He
		Biochim Biophys Acta Mol Basis Dis; Pyrazole derivative Z10 ameliorates acute pancreatitis by inhibiting the ERK/Ddt pathway; Authors - Wenying Zeng
mouse	WB,IHC	Int Immunopharmacol; Gastrodin ameliorates acute pancreatitis by modulating macrophage inflammation cascade via inhibition the p38/NF-κB pathway; Authors - Yalan Jiang
mouse	IHC	Mol Biol Cell; Vacuolization of mucolipidosis type II mouse exocrine gland cells represents accumulation of autolysosomes.; Authors - Boonen Marielle M
mouse	IF	Biomed Pharmacother; Serotonin-RhoA/ROCK axis promotes acinar-to-ductal metaplasia in caerulein-induced chronic pancreatitis.; Authors - Xufeng Tao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Gabriele (Verified Customer) (07-18-2025)

The antibody works very well both in WB (1:5000, 5% milk) and IF (1:200, 5% BSA) on HEK293T over-expressing human AMY2A 48h post-transfection vs not-treated control

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: WB: 1:5000 - IF: 1:200
• Cell Tissue Type: HEK293T

Amylase Alpha Antibody Western Blot,Immunofluorescence validation (WB: 1:5000 - IF: 1:200 dilution) in HEK293T (Cat no:12540-1-AP)

FH

balawant (Verified Customer) (08-06-2022)

This antibody is working great and need 1:20000 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:20000
• Cell Tissue Type: Intestinal tissue and colon cancer cell line

FH

Susmita (Verified Customer) (06-13-2022)

This is an excellent antibody for IHC and WB application

• Applications: Western Blot
• Primary Antibody Dilution: WB: 1: 4000, IHC 1: 400
• Cell Tissue Type: PC cell line PC tissue

FH

Iram (Verified Customer) (03-11-2022)

Very good antibody for western

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Colon cancer cells

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