Anticorps Polyclonal de lapin anti-Amylase Alpha

Amylase Alpha Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

Avis (3)

N° de cat : 12540-1-AP

Synonymes

Alpha amylase 2B, AMY2, AMY2B, Amylase Alpha, Amylase, alpha, amylase, alpha 2B (pancreatic), Carcinoid alpha amylase

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Galerie de données de validation

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  WB analysis of mouse pancreas using 12540-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12540-1-AP (Amylase, alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 12540-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12540-1-AP (Amylase, alpha antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12540-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12540-1-AP (Amylase, alpha antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu pancréatique de souris,
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 4 publications below
• IF: See 5 publications below

Informations sur le produit

12540-1-AP cible Amylase Alpha dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Amylase Alpha Protéine recombinante Ag3260
• Nom complet: amylase, alpha 2B (pancreatic)
• Masse moléculaire calculée: 511 aa, 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC011179
• Symbole du gène: AMY2B
• Identification du gène (NCBI): 280
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AMY2B(alpha-amylase 2B) belongs to the glycosyl hydrolase 13 family. This protein is involved in the endohydrolysis of 1,4-alpha-glucosidic linkages in oligosaccharides and polysaccharides. The full length 58 kDa protein has a signal peptide with 15 amino acids. Human amylases (Amy1, Amy2A, and Amy2B) commonly have two potential N-glycosylation sites (N427 and N476) in their C-terminal region (S Takashima, J Amano, 2012). 12540-1-AP can recognize both AMY2A and AMY2B.

Publications

Species	Application	Title
mouse	IF	Cancer Lett; SULF2 enhances GDF15-SMAD axis to facilitate the initiation and progression of pancreatic cancer.; Authors - Ruizhe He
mouse	IHC	Mol Biol Cell; Vacuolization of mucolipidosis type II mouse exocrine gland cells represents accumulation of autolysosomes.; Authors - Boonen Marielle M
mouse	IF	Biomed Pharmacother; Serotonin-RhoA/ROCK axis promotes acinar-to-ductal metaplasia in caerulein-induced chronic pancreatitis.; Authors - Xufeng Tao
mouse	IF	Eur J Pharmacol; Berberine inhibits pancreatic intraepithelial neoplasia by inhibiting glycolysis via the adenosine monophosphate -activated protein kinase pathway.; Authors - Mengmeng Liu
human	IHC	BMC Gastroenterol; Clinicopathological characteristics of pancreatic acinar cell metaplasia associated with Helicobacter pylori infection.; Authors - Takafumi Fuchino
human,rat	IF	iScience; Alterations in histology of the aging salivary gland and correlation with the glandular inflammatory microenvironment; Authors - Ning Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

balawant (Verified Customer) (08-06-2022)

This antibody is working great and need 1:20000 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:20000
• Cell Tissue Type: Intestinal tissue and colon cancer cell line

FH

Susmita (Verified Customer) (06-13-2022)

This is an excellent antibody for IHC and WB application

• Applications: Western Blot
• Primary Antibody Dilution: WB: 1: 4000, IHC 1: 400
• Cell Tissue Type: PC cell line PC tissue

FH

Iram (Verified Customer) (03-11-2022)

Very good antibody for western

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Colon cancer cells